Callose (beta-1,3 glucan) separates developing pollen grains, preventing their underlying walls (exine) from fusing. The pollen tubes that transport sperm to female gametes also contain callose, both in their walls as well as in the plugs that segment growing tubes. Mutations in CalS5, one of several Arabidopsis beta-1,3 glucan synthases, were previously shown to disrupt callose formation around developing microspores, causing aberrations in exine patterning, degeneration of developing microspores, and pollen sterility. Here, we describe three additional cals5 alleles that similarly alter exine patterns, but instead produce fertile pollen. Moreover, one of these alleles (cals5-3) resulted in the formation of pollen tubes that lacked callose walls and plugs. In self-pollinated plants, these tubes led to successful fertilization, but they were at a slight disadvantage when competing with wild type. Contrary to a previous report, these results demonstrate that a structured exine layer is not required for pollen development, viability or fertility. In addition, despite the presence of callose-enriched walls and callose plugs in pollen tubes, the results presented here indicate that callose is not required for pollen tube functions.
Premature dissolution of the microsporocyte callose wall causes male sterility in transgenic tobacco
A conditional sterile mutation eliminates surface components from Arabidopsis pollen and disrupts cell signaling during fertilization
Tetrad pollen formation in quartet mutants of Arabidopsis thaliana is associated with persistence of pectic polysaccharides of the pollen mother cell wall
DEX1, a novel plant protein, is required for exine pattern formation during pollen development in Arabidopsis
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Microspore separation in the quartet 3 mutants of Arabidopsis is impaired by a defect in a developmentally regulated polygalacturonase required for pollen mother cell wall degradation
Disruption of the novel plant protein NEF1 affects lipid accumulation in the plastids of the tapetum and exine formation of pollen, resulting in male sterility in Arabidopsis thaliana
Callose synthase (CalS5) is required for exine formation during microgametogenesis and for pollen viability in Arabidopsis
Precocious pollen germination in Arabidopsis plants with altered callose deposition during microsporogenesis
AtbZIP34 is required for Arabidopsis pollen wall patterning and the control of several metabolic pathways in developing pollen
UDP-glucose pyrophosphorylase is rate limiting in vegetative and reproductive phases in Arabidopsis thaliana
RUPTURED POLLEN GRAIN1, a member of the MtN3/saliva gene family, is crucial for exine pattern formation and cell integrity of microspores in Arabidopsis
The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth
CYP704B1 is a long-chain fatty acid omega-hydroxylase essential for sporopollenin synthesis in pollen of Arabidopsis
LAP5 and LAP6 encode anther-specific proteins with similarity to chalcone synthase essential for pollen exine development in Arabidopsis
ATP-binding cassette transporter G26 is required for male fertility and pollen exine formation in Arabidopsis
Callose synthase GSL7 is necessary for normal phloem transport and inflorescence growth in Arabidopsis
The cell wall of the Arabidopsis pollen tube--spatial distribution, recycling, and network formation of polysaccharides
GLUCAN SYNTHASE-LIKE8 and STEROL METHYLTRANSFERASE2 are required for ploidy consistency of the sexual reproduction system in Arabidopsis
CYCLIN-DEPENDENT KINASE G1 is associated with the spliceosome to regulate CALLOSE SYNTHASE5 splicing and pollen wall formation in Arabidopsis
Callose plug deposition patterns vary in pollen tubes of Arabidopsis thaliana ecotypes and tomato species
Developmental evolution of flowering plant pollen tube cell walls: callose synthase (CalS) gene expression patterns
Multiple BiP genes of Arabidopsis thaliana are required for male gametogenesis and pollen competitiveness
Arabidopsis RPG1 is important for primexine deposition and functions redundantly with RPG2 for plant fertility at the late reproductive stage
LAP3, a novel plant protein required for pollen development, is essential for proper exine formation
Localization and Quantification of Callose in the Streptophyte Green Algae Zygnema and Klebsormidium: Correlation with Desiccation Tolerance
Dual function of Arabidopsis glucan synthase-like genes GSL8 and GSL10 in male gametophyte development and plant growth
Transcriptional characteristics and differences in Arabidopsis stigmatic papilla cells pre- and post-pollination
GLUCAN SYNTHASE-LIKE 5 (GSL5) plays an essential role in male fertility by regulating callose metabolism during microsporogenesis in rice
The Arabidopsis LFR gene is required for the formation of anther cell layers and normal expression of key regulatory genes
Arabidopsis galacturonosyltransferase (GAUT) 13 and GAUT14 have redundant functions in pollen tube growth
Functional characterization and expression of GASCL1 and GASCL2, two anther-specific chalcone synthase like enzymes from Gerbera hybrida
Evolutionary history of callose synthases in terrestrial plants with emphasis on proteins involved in male gametophyte development
Correlation between pollen aperture pattern and callose deposition in late tetrad stage in three species producing atypical pollen grains
Proteomic and biochemical evidence links the callose synthase in Nicotiana alata pollen tubes to the product of the NaGSL1 gene
Microtubule involvement in the deposition of radial fibrillar callose arrays in stomata of the fern Asplenium nidus L
Development of the Mitsucal computer system to identify causal mutation with a high-throughput sequencer
WBC27, an adenosine tri-phosphate-binding cassette protein, controls pollen wall formation and patterning in Arabidopsis
Ultrastructural characterization of exine development of the transient defective exine 1 mutant suggests the existence of a factor involved in constructing reticulate exine architecture from sporopollenin aggregates
Biosynthesis of callose and cellulose by detergent extracts of tobacco cell membranes and quantification of the polymers synthesized in vitro
Genome-wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis)
The Arabidopsis Exine Formation Defect (EFD) gene is required for primexine patterning and is critical for pollen fertility
Defective Pollen Wall 3 (DPW3), a novel alpha integrin-like protein, is required for pollen wall formation in rice
A protocol for combining fluorescent proteins with histological stains for diverse cell wall components
The Quantitative Basis of the Arabidopsis Innate Immune System to Endemic Pathogens Depends on Pathogen Genetics
Genome wide transcriptome analysis reveals vital role of heat responsive genes in regulatory mechanisms of lentil (Lens culinaris Medikus)
Molecular control of the glucan synthase-like protein NaGSL1 and callose synthesis during growth of Nicotiana alata pollen tubes
BcMF9, a novel polygalacturonase gene, is required for both Brassica campestris intine and exine formation
Role of aromatic aldehyde synthase in wounding/herbivory response and flower scent production in different Arabidopsis ecotypes
Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation
Defective Microspore Development 1 is required for microspore cell integrity and pollen wall formation in rice
Down-regulation of the glucan synthase-like 6 gene (HvGsl6) in barley leads to decreased callose accumulation and increased cell wall penetration by Blumeria graminis f. sp. hordei
Novel localization of callose in the spores of Physcomitrella patens and phylogenomics of the callose synthase gene family
Evolution of Cell Wall Polymers in Tip-Growing Land Plant Gametophytes: Composition, Distribution, Functional Aspects and Their Remodeling
Identification of genes involved in male sterility in wheat (Triticum aestivum L.) which could be used in a genic hybrid breeding system
First Steps in the Successful Fertilization of Rice and Arabidopsis : Pollen Longevity, Adhesion and Hydration
Distinct Differentiation Characteristics of Endothelium Determine Its Ability to Form Pseudo-Embryos in Tomato Ovules
Characterisation of BcMF10, a novel gene involved in pollen wall development of Brassica rapa ssp. chinensis
Pollen wall development in Hydrangea bretschneiderii Dippel. (Hydrangeaceae): advanced interpretation through physical input, with in vitro experimental verification
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