Callose (beta-1,3 glucan) is essential for Arabidopsis pollen wall patterning, but not tube growth

BMC Plant Biology
Shuh-Ichi NishikawaDaphne Preuss


Callose (beta-1,3 glucan) separates developing pollen grains, preventing their underlying walls (exine) from fusing. The pollen tubes that transport sperm to female gametes also contain callose, both in their walls as well as in the plugs that segment growing tubes. Mutations in CalS5, one of several Arabidopsis beta-1,3 glucan synthases, were previously shown to disrupt callose formation around developing microspores, causing aberrations in exine patterning, degeneration of developing microspores, and pollen sterility. Here, we describe three additional cals5 alleles that similarly alter exine patterns, but instead produce fertile pollen. Moreover, one of these alleles (cals5-3) resulted in the formation of pollen tubes that lacked callose walls and plugs. In self-pollinated plants, these tubes led to successful fertilization, but they were at a slight disadvantage when competing with wild type. Contrary to a previous report, these results demonstrate that a structured exine layer is not required for pollen development, viability or fertility. In addition, despite the presence of callose-enriched walls and callose plugs in pollen tubes, the results presented here indicate that callose is not required for pollen tube functions.


Nov 18, 2000·American Journal of Botany·R C JacksonW F Chissoe
Apr 12, 2001·Plant Physiology·Monika Susanne DoblinSteve Read
Jun 14, 2003·Plant Physiology·David Honys, David Twell
Aug 2, 2003·Science·José M AlonsoJoseph R Ecker
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Loverine P. Taylor, Peter K. Hepler
Apr 13, 2004·The Plant Cell·Anna F EdlundDaphne Preuss
Apr 22, 2005·The Plant Journal : for Cell and Molecular Biology·Xiaoyun DongDesh Pal S Verma


Sep 30, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Cecile M SanoThomas W Jacobs
Jul 9, 2011·Sexual Plant Reproduction·Ji-Feng MaXian Sheng Zhang
Dec 1, 2010·Protoplasma·Raul ZavalievVitaly Citovsky
Aug 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Joseph H Williams
Jan 1, 2011·AoB Plants·Simon WallaceDavid J Beerling
Mar 27, 2009·Journal of Experimental Botany·Zoe A Wilson, Dabing Zhang
Sep 10, 2008·Plant & Cell Physiology·Toshiya SuzukiSumie Ishiguro
Apr 16, 2009·Plant & Cell Physiology·Meng MengLeszek A Kleczkowski
Sep 19, 2006·Plant Physiology·Kirk E FrancisGregory P Copenhaver
Oct 31, 2006·Plant Physiology·Georgia DrakakakiNatasha Raikhel
Feb 12, 2009·The Plant Cell·Amy L Szumlanski, Erik Nielsen
May 1, 2008·Annual Review of Plant Biology·Alice Y Cheung, Hen-Ming Wu
Aug 2, 2011·BMC Plant Biology·Chinnoi Law, Christopher Exley
Nov 7, 2013·Plant Reproduction·Yongxian LuMatthew M S Evans
Feb 4, 2014·Plant & Cell Physiology·Daisuke MaruyamaShuh-Ichi Nishikawa
Feb 1, 2011·Annual Review of Plant Biology·Tohru Ariizumi, Kinya Toriyama
Dec 25, 2009·Sexual Plant Reproduction·Anna A DobritsaRobert J Swanson
Oct 17, 2015·International Journal of Molecular Sciences·Sergey BombinKatrina M Ramonell
Oct 12, 2015·Plant Reproduction·Thomas J MusielakMartin Bayer
Mar 5, 2008·The Plant Journal : for Cell and Molecular Biology·Armin TöllerPaul Schulze-Lefert
Mar 10, 2011·Plant Signaling & Behavior·Bo Xie, Zonglie Hong
Oct 10, 2009·Plant Signaling & Behavior·Xiong-Yan Chen, Jae-Yean Kim
Nov 4, 2010·Plant Signaling & Behavior·Basil Galatis, Panagiotis Apostolakos
Sep 28, 2014·Plant & Cell Physiology·Minako SumiyoshiHiroaki Iwai
Dec 24, 2010·The Plant Journal : for Cell and Molecular Biology·Bo XieZonglie Hong
Dec 11, 2008·The Plant Journal : for Cell and Molecular Biology·Knut ThieleFarhah F Assaad
Jun 8, 2014·Phytochemistry·Teagen D QuilichiniCarl J Douglas
Jun 24, 2016·American Journal of Botany·Anna F EdlundDaphne Preuss
Aug 2, 2007·The Plant Journal : for Cell and Molecular Biology·Lynette BrownfieldAntony Bacic
Apr 14, 2009·Cell Motility and the Cytoskeleton·Panagiotis ApostolakosBasil Galatis
Nov 25, 2017·The Plant Journal : for Cell and Molecular Biology·Robertas UrsacheNiko Geldner
May 15, 2020·PLoS Genetics·Shuang-Xi XiongYue Lou
Sep 30, 2020·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Asja Radja
Feb 3, 2011·The Plant Journal : for Cell and Molecular Biology·Michael GutensohnNatalia Dudareva
Feb 6, 2014·The Plant Journal : for Cell and Molecular Biology·Yoko Mizuta, Tetsuya Higashiyama
Oct 29, 2017·BMC Plant Biology·Tohnyui Ndinyanka FabriceChristoph Ringli
May 12, 2020·The Plant Journal : for Cell and Molecular Biology·Lijun RenZhukuan Cheng
Dec 25, 2015·Journal of Integrative Plant Biology·Xiao-Ying DouDe Ye
Apr 20, 2010·Annals of Botany·Nina I GabarayevaJohn R Rowley
Jan 10, 2019·Plants·Michael KravchikTzahi Arazi
Dec 22, 2019·International Journal of Molecular Sciences·Inna ChabanElena Smirnova

Related Concepts

GSL1 protein, Arabidopsis
Genome Mapping
Male Gametophytes
Genes, Plant
Arabidopsis thaliana <plant>

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.