Callus culture and plantlet regeneration in date palm (Phoneix dactylifera L.): an important horticultural cash crop for arid and semi-arid horticulture.

Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology
Mangal Singh RathoreShahrukh A Siddiqui

Abstract

Phoneix dactylifera L. commonly called date palm is a highly valuable horticultural cash crop for arid and semi-arid regions. The availability of offshoots and their survival during plantation are major concern. Being dioecious tree, seed propagation in date palm do not produce true-to-type offspring and tissue culture propagation is the only viable option to supply quality-planting propagules. Hereby, we report callus culture and plantlet regeneration in female date palm using in vitro-derived adventitious shoot bud tissues as explants. Explants (89.33 ± 2.67%) produced callus culture on 0.8% agar-gelled Murashige and Skoog's basal medium containing 100.0 mg l-1 each polyvinylpyrrolidone, ascorbic acid and glutamine, 50.0 mg l-1 each citric acid, adenine sulphate and l-arginine as additives, 0.1% activated charcoal (AC), 100 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D) and 3.0 mg l-1 2-isopentenyladenine (2-iP). Callus culture were amplified on medium containing 3.0 mg l-1 2-iP along with 50 mg l-1 2,4-D for 2 passages and 10 mg l-1 2,4-D for 2 passages. Cultures grew moderately, organized and subsequently regenerated into shoot bud like structures during gradual transfer from medium containing higher concentration of 2,4-D t...Continue Reading

References

Apr 6, 2001·Biotechnology and Bioengineering·M J CorrellP J Weathers
Nov 5, 2008·Plant Molecular Biology·Hidehiro Fukaki, Masao Tasaka
Sep 3, 2011·Applied Biochemistry and Biotechnology·Mangal Singh RathoreN S Shekhawat
Apr 1, 2010·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Nitish KumarNaraynan Subhash
Jul 29, 2018·Applied Microbiology and Biotechnology·Saikat GantaitSandeep Kumar Verma

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