Calmodulin 1 Regulates Senescence and ABA Response in Arabidopsis

Frontiers in Plant Science
Cheng DaiJune M Kwak

Abstract

Cellular calcium acts as a second messenger and regulates diverse developmental events and stress responses. Cytosolic calcium has long been considered as an important regulator of senescence, however, the role of Ca2+ in plant senescence has remained elusive. Here we show that the Calmodulin 1 (CaM1) gene, which encodes Ca2+-binding protein calmodulin 1, positively regulates leaf senescence in Arabidopsis. Yellowing of leaves, accumulation of reactive oxygen species (ROS), and expression of the senescence-associated gene 12 (SAG12) were significantly enhanced in CaM1 overexpression plants. In contrast, abscisic acid (ABA)-triggered ROS production and stomatal closure were reduced in amiRNA-CaM1 plants. We found a positive-feedback regulation loop among three signaling components, CaM1, RPK1, and RbohF, which physically associate with each other. RPK1 positively regulates the expression of the CaM1 gene, and the CaM1 protein, in turn, up-regulates RbohF gene expression. Interestingly, the expression of CaM1 was down-regulated in rbohD, rbohF, and rbohD/F mutants. We show that CaM1 positively regulates ROS production, leaf senescence, and ABA response in Arabidopsis.

References

Jun 17, 1998·The Plant Journal : for Cell and Molecular Biology·M A TorresJ D Jones
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Oct 26, 1999·The Journal of Biological Chemistry·V S ReddyA S Reddy
Jul 8, 2000·Trends in Cell Biology·D Chin, A R Means
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Miguel Angel TorresJonathan D G Jones
Nov 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Radhika DesikanSteven Neill
Apr 2, 2003·Current Opinion in Plant Biology·Malla Padidam
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Raymond E. Zielinski
Jan 6, 2005·Genome Research·Alex AdaiVenkatesan Sundaresan
Apr 5, 2005·Nature Genetics·Markus SchmidJan U Lohmann
Jul 19, 2005·Trends in Plant Science·Elizabeth McCormackJanet Braam
Mar 15, 2006·The Plant Cell·Rebecca SchwabDetlef Weigel
Sep 1, 1973·Plant Physiology·B W Poovaiah, A C Leopold
Aug 1, 1992·Plant Physiology·C M Chou, C H Kao
Sep 12, 2006·The Plant Journal : for Cell and Molecular Biology·Radhika DesikanSteven J Neill
Dec 21, 2006·Annual Review of Plant Biology·Pyung Ok LimHong Gil Nam
Jan 26, 2008·The Journal of Biological Chemistry·Yoko OgasawaraKazuyuki Kuchitsu
Jan 22, 2010·The Journal of Biological Chemistry·Yuriko OsakabeKazuko Yamaguchi-Shinozaki
Mar 3, 2010·Annual Review of Plant Biology·Antony N DoddDale Sanders
Aug 24, 2010·Aging·Arnaud Vigneron, Karen H Vousden
Sep 10, 2014·Current Opinion in Plant Biology·Leonie Steinhorst, Jörg Kudla
Dec 30, 2014·Biochimica Et Biophysica Acta·Romy Schmidt, Jos H M Schippers
Feb 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Yang ZhaoJian-Kang Zhu
Mar 26, 2016·Frontiers in Plant Science·Benoît RantyChristian Mazars
May 21, 2016·Frontiers in Plant Science·Ina SaxenaZhong Chen
Jul 2, 2016·Current Opinion in Plant Biology·Kai H Edel, Jörg Kudla

❮ Previous
Next ❯

Citations

Jan 13, 2019·International Journal of Molecular Sciences·Muhammad Asad Ullah AsadFangmin Cheng
Nov 7, 2019·BMC Plant Biology·Worawat YuenyongTeerapong Buaboocha
Aug 18, 2021·Biochemical and Biophysical Research Communications·Yun ShangKyoung Hee Nam

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
Assay
PCR

Software Mentioned

Image J
SmartGrain
Adobe Photoshop
TotalLab100
MEGA5

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.