PMID: 6403556Apr 1, 1983Paper

Calmodulin antagonists inhibit secretion in Paramecium

The Journal of Cell Biology
R S GarofaloB H Satir

Abstract

Secretion in Paramecium is Ca2+-dependent and involves exocytic release of the content of the secretory organelle, known as the trichocyst. The content, called the trichocyst matrix, undergoes a Ca2+-induced reordering of its paracrystalline structure during release, and we have defined three stages in this expansion process. The stage I, or fully condensed trichocyst, is the 4 microns-long membrane-bounded form existing prior to stimulation. Stage II, the partially expanded trichocyst, we define as an intermediate stage in the transition, preceding stage III, the fully expanded extruded form which is a 20-40 microns-long needlelike structure. These stages have been used to assay the effects of trifluoperazine (TFP) and W-7, calmodulin (CaM) antagonists, on trichocyst matrix expansion in vivo. TFP and W-7 are shown to reversibly block matrix release induced by picric acid. Ultra-structural examination reveals that one effect of this inhibition is reflected in the organelles themselves, which are prevented from undergoing the stage I-stage II transition by preincubation in 14 microM TFP or 35 microM W-7 before fixation. This inhibition of expansion by TFP can be moderated but not abolished by high extracellular Ca2+ (5 mM). The ...Continue Reading

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Citations

Nov 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M L MuńozR Tovar
Feb 13, 1992·European Journal of Pharmacology·J M TrifaróA Rodríguez Del Castillo
Aug 1, 1987·The Journal of Cell Biology·K SandvigB van Deurs
Jul 1, 1983·The Journal of Cell Biology·D M Gilligan, B H Satir
Nov 1, 1983·The Journal of Cell Biology·D Bar-Sagi, J Prives
Jun 29, 1990·European Journal of Protistology·B Haacke-BellH Plattner
Jan 8, 2008·European Journal of Protistology·Soichiro Kakuta, Toshinobu Suzaki
Jul 1, 1989·The Journal of protozoology·B H Satir
Apr 1, 1988·Journal of Cellular Biochemistry·B H SatirT J Murtaugh
Nov 1, 1988·Journal of Neurochemistry·S Fournier, J M Trifaró
Apr 1, 1984·Journal of Cellular Physiology·A SundanS Olsnes
Feb 16, 2000·Biochemical and Biophysical Research Communications·P P LemonsS W Whiteheart
Aug 24, 2021·Frontiers in Molecular Neuroscience·Renhao XueHuisheng Liu

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