PMID: 2123288Sep 3, 1990Paper

Calmodulin binds to a tubulin binding site of the microtubule-associated protein tau

Molecular and Cellular Biochemistry
R PadillaJesus Avila

Abstract

Previous studies have demonstrated that the microtubule-associated proteins MAP-2 and tau interact selectively with common binding domains on tubulin defined by the low-homology segments alpha (430-441) and beta (422-434). It has been also indicated that the synthetic peptide VRSKIGSTENLKHQPGGG corresponding to the first tau repetitive sequence represents a tubulin binding domain on tau. The present studies show that the calcium-binding protein calmodulin interacts with a tubulin binding site on tau defined by the second repetitive sequence VTSKCGSLGNIHHKPGGG. It was shown that both tubulin and calmodulin bind to tau peptide-Sepharose affinity column. Binding of calmodulin occurs in the presence of 1 mM Ca 2+ and it can be eluted from the column with 4 mM EGTA. These findings provide new insights into the regulation of microtubule assembly, since Ca2+/calmodulin inhibition of tubulin polymerization into microtubules could be mediated by the direct binding of calmodulin to tau, thus preventing the interaction of this latter protein with tubulin.

Citations

Jan 16, 1999·Journal of Neuroscience Research·L S Yang, H Ksiezak-Reding
Apr 7, 1993·Molecular and Cellular Biochemistry·M BillgerM Wallin
Jan 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·G AknerJ A Gustafsson
Oct 31, 1998·Brain Research. Molecular Brain Research·M L SmithJ R Slemmon
Jul 5, 2012·Cold Spring Harbor Perspectives in Medicine·Eva-Maria Mandelkow, Eckhard Mandelkow
Oct 1, 1995·European Journal of Biochemistry·L S Yang, H Ksiezak-Reding
May 5, 2011·World Journal of Biological Chemistry·Elena Gomez de Barreda, Jesús Avila
Sep 1, 1993·Journal of Neurochemistry·D M MoragaR B Maccioni
Jul 19, 2015·Neuroscience Letters·Xiaona ZhangAnmu Xie
Mar 28, 2012·Annals of Neurology·Nathan PankratzUNKNOWN PD GWAS Consortium
Jan 7, 2015·Neurobiology of Aging·Zhen-hua LiuBei-sha Tang
May 3, 2013·Neurobiology of Aging·Chin-Hsien LinRuey-Meei Wu
Dec 24, 2014·Parkinsonism & Related Disorders·Jian-Yong WangJian-Hong Zhu
Mar 31, 2015·Journal of Alzheimer's Disease : JAD·Danton H O'DayMichael A Myre
Jul 22, 2008·Biochimica Et Biophysica Acta·Da-yu YuQun Wei
May 3, 2011·Biochemical and Biophysical Research Communications·Elena Gómez de Barreda, Jesús Avila
Nov 8, 2016·Biochimica Et Biophysica Acta. Molecular Cell Research·María BerrocalAna M Mata
Nov 28, 2016·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Jun-Ying LiRong Peng
Dec 1, 1995·Cell Biochemistry and Function·J P HenríquezR B Maccioni
Jul 9, 2013·The Journal of Biological Chemistry·Julien LefèvrePatrick A Curmi
Jun 7, 2000·The Journal of Biological Chemistry·M HashiguchiH K Paudel
Oct 18, 2019·Frontiers in Aging Neuroscience·Nataliya I TrushinaRoland Brandt
May 6, 2015·The Journal of Biological Chemistry·Noelia Inés BurgardtMatthias Weiwad
May 16, 2019·International Journal of Molecular Sciences·Giacomo Gattoni, Graziella Bernocchi
Apr 6, 2000·The Journal of Biological Chemistry·K SobueH K Paudel
Apr 4, 2021·International Journal of Molecular Sciences·Tomasz BoczekLudmila Zylinska
Jun 3, 2021·International Journal of Molecular Sciences·Joana PoejoCarlos Gutierrez-Merino
Sep 1, 1991·Journal of Neuroscience Research·C W ScottC B Caputo
Aug 27, 2021·Metabolites·Alexander Myrka, Leslie Buck

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