Calmodulin-dependent protein kinase IV during T-cell development

Biochemical and Biophysical Research Communications
J KrebsP Kisielow

Abstract

In a primary cell culture system of fetal rat brain, the calmodulin-dependent protein-kinase IV (CaMKIV) could be induced by the thyroid hormone T3 in a time- and concentration-dependent manner, provided the tissue was excised not later than day 15 of gestation (E15) (Krebs et al., J. Biol. Chem. 271, 11055, 1996). We report here that in the fetal thymus CaMKIV could not be detected earlier than day 16 of gestation and that the expression of this enzyme was fully upregulated at day 18. In mouse fetal thymus organ culture (FTOC) of day 14 embryonic thymus, CaMKIV could not be detected, even after several days of culture if a minimal culture medium lacking fetal calf serum was used. However, after addition of fetal calf serum to the culture medium the expression of CaMKIV could be specifically induced. Furthermore, it could also be shown that during T-cell development in the adult murine thymus the expression of CaMKIV was tightly regulated. Taken together, these results demonstrate that the expression of CaMKIV, an enzyme involved in the regulation of Ca(2+)-dependent gene expression, is itself under stringent regulatory control during tissue development.

Citations

Jul 12, 2011·Nature Immunology·Helder I NakayaBali Pulendran
Dec 14, 2011·Current Opinion in HIV and AIDS·Helder I Nakaya, Bali Pulendran
Jan 17, 2016·Progress in Biophysics and Molecular Biology·Huma NazMd Imtaiyaz Hassan
Aug 21, 2013·Current Opinion in Immunology·Shai S Shen-Orr, David Furman
Aug 17, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kellie E CunninghamKevin P Mollen
Nov 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·V RamanT A Chatila
May 1, 2021·International Journal of Molecular Sciences·Kinga SałaciakKarolina Pytka
Jan 27, 1999·Biochimica Et Biophysica Acta·J Krebs

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