Calmodulin disruption impacts growth and motility in juvenile liver fluke

Parasites & Vectors
Erin M McCammickAaron G Maule

Abstract

Deficiencies in effective flukicide options and growing issues with drug resistance make current strategies for liver fluke control unsustainable, thereby promoting the need to identify and validate new control targets in Fasciola spp. parasites. Calmodulins (CaMs) are small calcium-sensing proteins with ubiquitous expression in all eukaryotic organisms and generally use fluctuations in intracellular calcium levels to modulate cell signalling events. CaMs are essential for fundamental processes including the phosphorylation of protein kinases, gene transcription, calcium transport and smooth muscle contraction. In the blood fluke Schistosoma mansoni, calmodulins have been implicated in egg hatching, miracidial transformation and larval development. Previously, CaMs have been identified amongst liver fluke excretory-secretory products and three CaM-like proteins have been characterised biochemically from adult Fasciola hepatica, although their functions remain unknown. In this study, we set out to investigate the biological function and control target potential of F. hepatica CaMs (FhCaMs) using RNAi methodology alongside novel in vitro bioassays. Our results reveal that: (i) FhCaMs are widely expressed in parenchymal cells thro...Continue Reading

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Citations

Sep 10, 2016·Journal of Helminthology·C Carmona, J F Tort
Feb 10, 2017·Journal of Molecular Modeling·Daniel Garcez S QuattrociocchiLeonardo Moreira da Costa
Aug 17, 2016·Biochemical Society Transactions·Charlotte M Thomas, David J Timson
Nov 5, 2019·Scientific Reports·Hicham HouhouSimone Haeberlein

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Datasets Mentioned

BETA
AY005475

Methods Mentioned

BETA
PCR
phosphotransferase
light microscopy
PCRs
motility assay
ELISA
Assay

Software Mentioned

Artemis
Real Time PCR Miner
tBLASTn
ImageJ

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