Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites.

The EMBO Journal
Helga GrötschMaria-Isabel Geli

Abstract

Myosins-I are conserved proteins that bear an N-terminal motor head followed by a Tail Homology 1 (TH1) lipid-binding domain. Some myosins-I have an additional C-terminal extension (C(ext)) that promotes Arp2/3 complex-dependent actin polymerization. The head and the tail are separated by a neck that binds calmodulin or calmodulin-related light chains. Myosins-I are known to participate in actin-dependent membrane remodelling. However, the molecular mechanisms controlling their recruitment and their biochemical activities in vivo are far from being understood. In this study, we provided evidence suggesting the existence of an inhibitory interaction between the TH1 domain of the yeast myosin-I Myo5 and its C(ext). The TH1 domain prevented binding of the Myo5 C(ext) to the yeast WIP homologue Vrp1, Myo5 C(ext)-induced actin polymerization and recruitment of the Myo5 C(ext) to endocytic sites. Our data also indicated that calmodulin dissociation from Myo5 weakened the interaction between the neck and TH1 domains and the C(ext). Concomitantly, calmodulin dissociation triggered Myo5 binding to Vrp1, extended the myosin-I lifespan at endocytic sites and activated Myo5-induced actin polymerization.

References

Aug 1, 1992·The Journal of Cell Biology·S E Brockerhoff, T N Davis
Jan 1, 1991·Cell Motility and the Cytoskeleton·D WesselsD R Soll
Jan 1, 1991·Methods in Enzymology·V DulicH Riezman
Jan 1, 1991·Annual Review of Physiology·T D PollardH G Zot
Jun 14, 1991·Cell·J R GeiserT N Davis
Aug 1, 1990·The Journal of Cell Biology·S M HaydenM S Mooseker
Aug 17, 1989·Nature·R J Adams, T D Pollard
Oct 1, 1989·The Journal of Cell Biology·H MiyataE D Korn
Jul 11, 1988·Nucleic Acids Research·W J DowerC W Ragsdale
Mar 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E van Tuinen, H Riezman
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Dec 1, 1995·The Journal of Cell Biology·K D NovakM A Titus
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·A DurrbachE Coudrier
Feb 10, 1997·The Journal of Cell Biology·K D Novak, M A Titus
Jan 1, 1997·Annual Review of Biophysics and Biomolecular Structure·J Kuriyan, D Cowburn
May 8, 1999·Molecular Biology of the Cell·G RaposoE Coudrier
Jan 29, 2000·The Journal of Cell Biology·T LechlerR Li
Sep 7, 2000·The Journal of Cell Biology·E M Neuhaus, T Soldati
Sep 20, 2000·The Journal of Cell Biology·R RohatgiM W Kirschner
Dec 12, 2001·Molecular Biology of the Cell·M N CordonnierE Coudrier
Sep 11, 2002·The Journal of Biological Chemistry·Nanyun TangE Michael Ostap
Nov 14, 2002·Molecular Biology of the Cell·Fatima-Zahra IdrissiM Isabel Geli
Aug 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Takashi IshikawaAlasdair C Steven
Sep 3, 2004·Current Biology : CB·Gudrun A Jonsdottir, Rong Li

❮ Previous
Next ❯

Citations

Dec 24, 2011·Nature Cell Biology·Douglas R BoettnerSandra K Lemmon
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Fatima-Zahra IdrissiMaría Isabel Geli
Jan 28, 2012·The Journal of Chemical Physics·L Dupuis, Normand Mousseau
Sep 5, 2013·Cellular and Molecular Life Sciences : CMLS·Fatima-Zahra Idrissi, María Isabel Geli
Jun 6, 2012·Annual Review of Biochemistry·Olivia L MoorenJohn A Cooper
Sep 3, 2011·Traffic·Maite Vidal-QuadrasFrancesc Tebar
Oct 1, 2014·Developmental Cell·Isabel María Fernández-GolbanoMaría Isabel Geli
Sep 23, 2011·Biochemical Society Transactions·Jonathan GiblinMaría Isabel Geli
Nov 12, 2015·Developmental Cell·Eric B LewellynDavid G Drubin
Jun 13, 2015·PloS One·Erik VerschuerenBen Distel
Aug 9, 2019·Journal of Cell Science·Cameron Dale MacQuarrieVladimir Sirotkin
Jul 21, 2021·The Journal of Cell Biology·Javier Encinar Del DedoMaria Isabel Geli
Aug 17, 2011·Journal of Proteome Research·Simone S LiMarc R Wilkins

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.