Calmodulin in starfish oocytes. II. Trypsin treatment suppresses the trifluoperazine-sensitive step

Developmental Biology
L Meijer, P Guerrier

Abstract

The 1-methyladenine-induced oocyte maturation in starfish is reversibly inhibited by the anticalmodulin drug, trifluoperazine (TFP). However, when oocytes are exposed for 10 min to trypsin, they lose their sensitivity to TFP. Trypsin does not alter the length of the hormone-dependent period (1-methyladenine minimal contact time) or the 1-methyladenine concentration requirements. Trypsin-treated oocytes remain sensitive to other maturation inhibitors such as procaine, theophylline, caffeine, and D-600. Trypsin exposure modifies the protein pattern composition of the oocyte cortex (breakdown of a 140-kDa protein). TFP binding site localization was studied using fluorescence microscopy: in addition to a general diffuse fluorescence, staining is localized to probably acidic granules located in the cortex. Results are discussed in relation to calmodulin and plasma membrane calmodulin-dependent enzyme involvement in the stimulation of starfish oocyte maturation.

References

Nov 1, 1978·Archives of Biochemistry and Biophysics·M F PlishkerL A Goldsmith
Jun 20, 1978·Annals of the New York Academy of Sciences·P J Birckbichler, M K Patterson
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Mar 18, 1982·Biochimica Et Biophysica Acta·L Meijer, P Guerrier
Jan 1, 1984·International Review of Cytology·L Meijer, P Guerrier
May 1, 1982·Experimental Cell Research·M DoréeJ G Demaille
Mar 1, 1980·Archives of Biochemistry and Biophysics·M O Pinkett, W B Anderson

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Citations

Dec 1, 1983·Developmental Biology·L Meijer, P Guerrier
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·D M BermanA Coviello
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·D van HeemstD Zickler
Nov 1, 1984·The Journal of Cell Biology·A Eisen, G T Reynolds

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