PMID: 8584444Jan 1, 1996Paper

Calmodulin increases the initial rate of exocytosis in adrenal chromaffin cells

Pflügers Archiv : European journal of physiology
A V Kibble, R D Burgoyne

Abstract

The effect of calmodulin on exocytosis in bovine adrenal medullary chromaffin cells was examined by the use of patch-clamp capacitance recording. Calmodulin was dialysed into cells via the patch-pipette and cells stimulated by depolarisation. Following a test stimulation, cells were dialysed with a control or with a calmodulin containing buffer for 10 mins and then were stimulated at 2 min intervals thereafter. The inclusion of calmodulin in the pipette did not increase Ca2+ currents which instead decreased during dialysis. The presence of calmodulin, however, resulted in a 2-fold increase in the initial rate of exocytosis during the 10 min depolarisation step. These results demonstrate the utility of the patch-clamp capacitance technique for the examination of the effect of soluble proteins on exocytosis and in conjunction with previous work on permeabilised chromaffin cells suggest that calmodulin regulates late steps in Ca2+-dependent exocytosis.

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Citations

Dec 31, 1997·Neurochemistry International·K Langley, N J Grant
Jun 4, 2010·The Journal of Biological Chemistry·Jerome Di GiovanniMichael Seagar
Jul 30, 2002·The EMBO Journal·Stephanie QuetglasMichael Seagar
Feb 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Luc de HaroMichael Seagar
Mar 26, 1999·Cell Calcium·R D Burgoyne, A Morgan
Aug 17, 1999·Current Opinion in Cell Biology·A Mayer
Aug 14, 2003·Biochimica Et Biophysica Acta·Robert D Burgoyne, Michael J Clague
Aug 19, 2014·Journal of Medical Virology·Kelsey M HenriquezBruce Barrett
Jun 28, 2002·Physiological Reviews·Michael C Ashby, Alexei V Tepikin
Mar 21, 1997·The Journal of Biological Chemistry·M I ColomboP D Stahl
Mar 29, 2003·Physiological Reviews·Robert D Burgoyne, Alan Morgan
Aug 24, 2021·Frontiers in Molecular Neuroscience·Renhao XueHuisheng Liu

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