Calmodulin-mediated activation of Akt regulates survival of c-Myc-overexpressing mouse mammary carcinoma cells.

The Journal of Biological Chemistry
Tushar B DebRobert B Dickson

Abstract

c-Myc-overexpressing mammary epithelial cells are proapoptotic; their survival is strongly promoted by epidermal growth factor (EGF). We now demonstrate that EGF-induced Akt activation and survival in transgenic mouse mammary tumor virus-c-Myc mouse mammary carcinoma cells are both calcium/calmodulin-dependent. Akt activation is abolished by the phospholipase C-gamma inhibitor U-73122, by the intracellular calcium chelator BAPTA-AM, and by the specific calmodulin antagonist W-7. These results implicate calcium/calmodulin in the activation of Akt in these cells. In addition, Akt activation by serum and insulin is also inhibited by W-7. EGF-induced and calcium/calmodulin-mediated Akt activation occurs in both tumorigenic and non-tumorigenic mouse and human mammary epithelial cells, independent of their overexpression of c-Myc. These results imply that calcium/calmodulin may be a common regulator of Akt activation, irrespective of upstream receptor activator, mammalian species, and transformation status in mammary epithelial cells. However, only c-Myc-overexpressing mouse mammary carcinoma cells (but not normal mouse mammary epithelial cells) undergo apoptosis in the presence of the calmodulin antagonist W-7, indicating the vital ...Continue Reading

References

Jan 1, 1991·Journal of Neurochemistry·N YanagiharaE Miyamoto
Nov 1, 1990·Journal of Cellular Physiology·E M ValveriusD S Salomon
Jan 1, 1980·Annual Review of Biochemistry·C B KleeP G Richman
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K G DanielsonD Medina
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H HidakaT Nagata
Nov 24, 1995·Science·Z XiaM E Greenberg
Mar 24, 1995·Science·A Levitzki, A Gazit
Feb 28, 1994·Biochemical and Biophysical Research Communications·H MinamiH Hidaka
Aug 9, 1996·The Journal of Biological Chemistry·P KalimanA Zorzano
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·N N AhmedP N Tsichlis
Nov 14, 1997·The Journal of Biological Chemistry·J L JoyalD B Sacks
Aug 15, 1998·The Journal of Biological Chemistry·M BoschN Agell
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L C Cantley, B G Neel
Jun 15, 1999·Trends in Biochemical Sciences·T R Soderling
Jun 22, 1999·Molecular and Cellular Biology·N FilippaE Van Obberghen
Dec 2, 1999·Genes & Development·S R DattaM E Greenberg
Jan 27, 2000·Cell·D Hanahan, R A Weinberg
Aug 31, 2000·Cell Biochemistry and Function·J IchikawaT Kiyohara
Mar 30, 2001·The Journal of Biological Chemistry·T B DebG R Johnson
May 3, 2001·Frontiers in Bioscience : a Journal and Virtual Library·N NormannoD S Salomon
May 26, 2001·The Journal of Biological Chemistry·J P WhiteheadD E James

❮ Previous
Next ❯

Citations

Jul 1, 2008·Breast Cancer Research and Treatment·Christine M CoticchiaMichael D Johnson
Aug 26, 2006·Nature Clinical Practice. Nephrology·Vicente E Torres, Peter C Harris
Mar 10, 2006·Molecular Biology of the Cell·Yi WangZhixiang Wang
Nov 26, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Mi-kyung HwangSeong Hwan Kim
Feb 18, 2011·American Journal of Physiology. Cell Physiology·Tushar B DebMichael D Johnson
Jun 20, 2008·American Journal of Physiology. Cell Physiology·Tushar B DebMichael D Johnson
Aug 1, 2008·American Journal of Physiology. Renal Physiology·Trairak PisitkunMark A Knepper
Sep 16, 2006·Biological Chemistry·Jennifer J SchlezingerDavid H Sherr
Apr 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xinchun ShenRihe Liu
Feb 14, 2016·Expert Opinion on Therapeutic Targets·Ruth NussinovOzlem Keskin
Jul 3, 2015·Experimental and Therapeutic Medicine·Rui FengLiying Hao
Sep 15, 2007·Cell Calcium·Xinchun ShenRihe Liu
Dec 16, 2006·The Journal of Eukaryotic Microbiology·Mark E LasburyChao-Hung Lee
Sep 22, 2007·Journal of Thrombosis and Haemostasis : JTH·J C ReséndizR Lassila
Jul 14, 2010·Journal of Cellular Physiology·Ricardo ArmisénAndrés Stutzin
Jun 13, 2006·Cell Calcium·Christine M MisquittaAshok K Grover
Mar 18, 2015·Biochimica Et Biophysica Acta·Daniela SalasJuan C Sáez
Nov 6, 2013·Biochimica Et Biophysica Acta·Martin W Berchtold, Antonio Villalobo
Mar 31, 2015·Frontiers in Immunology·Xinxin WangYina Hsing Huang
Jan 30, 2015·Apoptosis : an International Journal on Programmed Cell Death·Marie-Laure BonnefondMonique N'Diaye
Jun 28, 2016·Cell Biology and Toxicology·Georgios DivolisPanagiota Papazafiri
Apr 28, 2017·International Journal of Molecular Sciences·Themmila KhamrangJoen-Rong Sheu
Dec 8, 2005·Journal of Lipid Research·Magdalena MaslowskaKatherine Cianflone
Feb 23, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chih-Hsuan HsiaKao-Chang Lin
Jul 17, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kaiyu YuanMajd Zayzafoon
May 17, 2018·Oncotarget·Camila Ramalho BonturiMaria Luiza Vilela Oliva
Apr 22, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mark R TannerChristine Beeton

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Breast Cancer Research and Treatment
Christine M CoticchiaMichael D Johnson
Proceedings of the National Academy of Sciences of the United States of America
Xinchun ShenRihe Liu
The Journal of Biological Chemistry
J L JoyalD B Sacks
© 2022 Meta ULC. All rights reserved