Calnexin expression does not enhance the generation of MHC class I-peptide complexes

European Journal of Immunology
S A PrasadJ R Bennink

Abstract

We investigated the requirement for calnexin in the biogenesis of MHC class I molecules. Mutant human cells lacking calnexin were infected with recombinant vaccinia viruses encoding mouse MHC class I molecules, Kd, Kb, Kk, Dd, Db, and Ld. Flow cytometry indicated that each of the six MHC class I allomorphs was transported to the cell surface at similar rates in calnexin-deficient cells and transfectants expressing calnexin. For Kb and Kd, the calnexin-independent biogenesis occurred regardless of whether the MHC class I molecules contained human or mouse beta 2-microglobulin. Also addressed was the effect of calnexin on the surface expression of Kb molecules bearing the immunodominant peptide from ovalbumin (OVA257-264). This was detected with a recently described monoclonal antibody specific for the Kb/peptide complex. Calnexin expression had no significant effect on the formation of Kb/peptide complexes generated from full-length OVA, cytosolic OVA257-264, or endoplasmic reticulum-targeted OVA257-264, which was expressed in the presence of the herpes simplex virus ICP47 protein to ensure detection of TAP-independent peptide-MHC class I complexes. Complementary results were obtained with TAP-independent formation of Kd/ peptid...Continue Reading

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Citations

Apr 5, 2005·Methods : a Companion to Methods in Enzymology·Marie-Eve PaquetDavid B Williams
Feb 27, 1999·Current Opinion in Immunology·P M van Endert
Nov 24, 1999·The Biochemical Journal·M MichalakM Opas
Oct 9, 2002·Molecular and Cellular Biology·Angela DenzelMichael J Owen
Jun 14, 2005·The Journal of Biological Chemistry·Michel PierenMaurizio Molinari
Dec 22, 1999·The Journal of Immunology : Official Journal of the American Association of Immunologists·C A PehJ McCluskey
Jul 8, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sebastian NeussAlexander Steinle
Jan 7, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sophie TourdotKeith G Gould
Jun 6, 2003·Biochimica Et Biophysica Acta·Kajsa Paulsson, Ping Wang

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