Caloric restriction improves endothelial dysfunction during vascular aging: Effects on nitric oxide synthase isoforms and oxidative stress in rat aorta

Experimental Gerontology
Michela ZanettiGianfranco Guarnieri

Abstract

Aging is characterized by activation of inducible over endothelial nitric oxide synthase (iNOS and eNOS), impaired antioxidant activity and increased oxidative stress, which reduces nitric oxide bioavailability and causes endothelial dysfunction. Caloric restriction (CR) blunts oxidative stress. We investigated whether CR impacts endothelial dysfunction in aging and the underlying mechanisms. Aortas from young (YC, 6 months of age) and old (OC, 24 months of age) rats ad-libitum fed and from old rats caloric-restricted for 3-weeks (OR, 26%) were investigated. Endothelium-dependent vasorelaxation was impaired in OC, associated with reduced eNOS and increased iNOS expression (P<0.05). Aortic nitrite was similar in OC and YC, but the contribution of calcium-independent NOS to total NOS activity was increased whereas that of calcium-dependent NOS was reduced (p≤0.0003). Plasma thiobarbituric acid-reactive substances (TBARS) were elevated in OC as well as aortic nitrotyrosine (P<0.05). Expression of manganese superoxide dismutase (MnSOD) and total SOD activity were impaired in OC (P<0.05 vs. YC), whereas copper-zinc (CuZn) SOD expression was similar in OC and YC. CR restored endothelial dysfunction in old rats, reduced iNOS expressio...Continue Reading

Citations

Apr 25, 2012·Naunyn-Schmiedeberg's Archives of Pharmacology·Noureddine Idris-Khodja, Valérie Schini-Kerth
Aug 16, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Edward P Weiss, Luigi Fontana
Oct 18, 2011·PloS One·Lina A ShehadehRoberto I Vazquez-Padron
Mar 30, 2016·Theriogenology·Maria Fernanda S ReghiniMarco Antonio Alvarenga
Aug 12, 2014·Science China. Life Sciences·Yue LiuDePei Liu
Nov 20, 2012·The Journal of Nutritional Biochemistry·Gianluca Gortan CappellariMichela Zanetti
Jul 20, 2012·Journal of Clinical Laboratory Analysis·Mahmut AbuhandanHatip Kılıc
Feb 18, 2014·Diabetes/metabolism Research and Reviews·Andreea SoarePaolo Pozzilli
Jul 16, 2013·Free Radical Biology & Medicine·Mariam El AssarLeocadio Rodríguez-Mañas
Jun 18, 2016·Nutrients·Concha F García-Prieto, María S Fernández-Alfonso
Oct 30, 2016·Experimental Gerontology·Miguel DiazMay Azzawi
Nov 30, 2016·Scientific Reports·Gianluca Gortan CappellariMichela Zanetti
Sep 20, 2016·The Journal of Physiology·Christopher R Martens, Douglas R Seals
Nov 10, 2015·Acta Physiologica·S W S Leung, P M Vanhoutte
Sep 14, 2018·Journal of Applied Physiology·Matthew J RossmanDouglas R Seals
May 25, 2013·Journal of Neuro-oncology·Yong-Sheng Jiang, Fu-Rong Wang
Dec 1, 2014·Advances in Physiology Education·Jessica R Santos-ParkerDouglas R Seals
Feb 15, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Ken ShinmuraIsao Ishii
Jun 26, 2020·Clinical Science·Matthew J RossmanDouglas R Seals
Oct 26, 2018·Circulation Research·Mahmoud AbdellatifGuido Kroemer
Jan 27, 2019·International Journal of Molecular Sciences·Michela ZanettiRocco Barazzoni
Jul 10, 2018·BioMed Research International·James Oluwagbamigbe FajemiroyeGustavo Rodrigues Pedrino
Jun 22, 2019·Oxidative Medicine and Cellular Longevity·Magbubah EssackEsma R Isenovic
Oct 17, 2020·Oxidative Medicine and Cellular Longevity·Andy W C ManNing Xia
Apr 10, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Danielle L KirkmanDavid G Edwards
Apr 2, 2018·Mechanisms of Ageing and Development·Mariam El AssarLeocadio Rodríguez-Mañas
Oct 17, 2017·Archives of Medical Research·Michela ZanettiRocco Barazzoni
Jul 6, 2021·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Christelle GuéréBérengère Fromy

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