Jan 3, 1977

Calorimetric studies on melting of tRNA Phe (yeast)

European Journal of Biochemistry
H J HinzP L Privalov

Abstract

The heat effects involved in thermal unfolding of tRNAPhe from yeast have been determined in various buffer systems by direct differential scanning calorimetry. Perfect reversibility of the melting process has been demonstrated for measurements in the absence of Mg2+ ions. The overall molar transition enthalpy, delta Ht = 298 +/- 15 kcal mol-1 (1247 +/- 63 kJ mol-1), has been shown to be independent of the NaCl concentration and the nature of the buffers used in this study. Delta Ht is identical in the presence and in the absence of Mg2+ ions within the margin of experimental error. This experimental result implies a vanishing or very small heat capacity change to be associated with melting. Decomposition of the calorimetrically determined complex transition curves, on the assumption that the experimental melting profile represents the sum of independent two-state transitions, results in five transitions which have been assigned to melting of different structural domains of the tRNA.

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Mentioned in this Paper

Buffers
Thermodynamics
Phenylalanine-Specific tRNA
Effects of Heat
RNA Denaturation
MT-TA gene
Triplet Codon-amino Acid Adaptor Activity
Transfer RNA
Calorimetry
Molar Tooth

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