Calpain 2 expression pattern and sub-cellular localization during mouse embryogenesis

The International Journal of Developmental Biology
Fabrice RaynaudMireille Rossel

Abstract

Regulation of migration and proliferation by calpain has been shown in various cell types; however, no data are available concerning calpain 2 (capn2) localization in embryonic tissues. Here, we report the expression pattern of capn2 during mouse embryonic development. Expression of the capn2 gene is observed throughout embryonic development. From ES cells and the 8-cell stage to late neurulation stages, CAPN2 is expressed in the cytoplasm and nuclear compartments, with a clear co-localisation with chromatin. Whole-mount in situ hybridization analysis from E8.5 to 14.5 stages indicates high levels of capn2 expression in the nervous system, heart and mesodermal tissues. Up-regulation is maintained during later developmental stages in proliferating cells and in precursor cells involved in muscle (myoblasts) or bone formation (chondrocytes). At later developmental stages, elevated mRNA levels coincided with CAPN2 nuclear localization in these cell types, while differentiated cells maintained cytoplasmic expression. This detailed analysis reveals dynamic expression: nuclear localization was associated either with active cell mitosis in embryonic stem cells and early developmental stages or with precursor cells later during organoge...Continue Reading

Citations

Jan 23, 2010·Chromosoma·Laura Magnaghi-JaulinFabrice Raynaud
Nov 19, 2010·Cellular and Molecular Life Sciences : CMLS·Shang-Min ChouTsai-Kun Li
Oct 19, 2010·European Journal of Medical Genetics·Jill A RosenfeldLisa G Shaffer
Sep 11, 2010·Tissue & Cell·E N MoudilouC Brun
Sep 16, 2009·Experimental Neurology·Yanzhang LiJames W Geddes
Aug 18, 2009·Archives of Oral Biology·T MatsunagaT Tachikawa
Jul 13, 2016·The Biochemical Journal·Lucía Rodríguez-FernándezElena R García-Trevijano
Feb 13, 2021·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Qianglai TanHua Wei
Jul 3, 2021·Frontiers in Molecular Biosciences·Michel BaudryXiaoning Bi
Aug 13, 2021·Scientific Reports·L Rodríguez-FernándezE R García-Trevijano

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