Calpain and caspase processing of caspase-12 contribute to the ER stress-induced cell death pathway in differentiated PC12 cells

Apoptosis : an International Journal on Programmed Cell Death
Juan A MartinezStephen F Larner

Abstract

Neuronal cell death after traumatic brain injury, Alzheimer's disease and ischemic stroke may in part be mediated through endoplasmic reticulum (ER) stress and unfolded protein response (UPR). UPR results in induction of molecular chaperone GRP78 and the ER-resident caspase-12, whose activation has been proposed to be mediated by calpain and caspase processing, although their relative contribution remains unclear. In this study we induced ER stress with thapsigargin (TG), and determined the activation profile of calpain-2, caspase-3, caspase-7, and caspase-12 by analyses of protein levels, corresponding substrates and breakdown products (BDP). Specific calpain and caspase activity was assessed by analysis of αII-spectrin BDP of 145 kDa (SBDP145), BDP of 150 kDa (SBDP150) and BDP of 120 kDa (SBDP120). Decrease in pro-calpain-2 protein and increased SBDP145 levels by 3 h after TG treatment indicated early calpain activity. Active caspase-7 (p20) increase occurred after 8 h, followed by concomitant up-regulation of active caspase-3 and SBDP120 after 24 h. In vitro digestion experiments supported that SBDP120 was exclusively generated by active caspase-3 and validated that kinectin and co-chaperone p23 were calpain and caspase-7 su...Continue Reading

References

Feb 10, 1997·FEBS Letters·M Van de CraenW Fiers
Jun 17, 1998·Genes & Development·V Cryns, J Yuan
Aug 26, 1998·The Journal of Biological Chemistry·K K WangJ S Morrow
Oct 15, 1998·FEBS Letters·T MachleidtM Krönke
Sep 22, 1999·European Journal of Biochemistry·P Korge, J N Weiss
Sep 24, 1999·Journal of Neuroscience Research·S L Chan, M P Mattson
Jan 13, 2000·Trends in Neurosciences·K K Wang
Jul 13, 2001·The Journal of Biological Chemistry·R V RaoD E Bredesen
Nov 10, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J K Newcomb-FernandezR L Hayes
Mar 29, 2002·The Journal of Biological Chemistry·Rammohan V RaoH Michael Ellerby
Jun 12, 2002·Cell Death and Differentiation·J F HarrimanR G Schnellmann
Sep 17, 2002·Cell Death and Differentiation·E FujitaT Momoi
Mar 4, 2003·Nature Reviews. Molecular Cell Biology·Lars Ellgaard, Ari Helenius
Oct 30, 2003·The Journal of Biological Chemistry·Nadia KorfaliWilliam C Earnshaw
Sep 15, 2004·Journal of Chemical Neuroanatomy·Takashi Momoi
Oct 12, 2004·FEBS Letters·Viviane Hoppe, Jürgen Hoppe
Apr 22, 2005·Apoptosis : an International Journal on Programmed Cell Death·W LeeI Mook-Jung
Jun 1, 2005·Bioorganic & Medicinal Chemistry·Yoshihisa ShirasakiMasayuki Nakamura
Nov 2, 2005·Annals of Clinical Biochemistry·Steven M HarwoodDavid A Allen
Jan 7, 2006·Cell Death and Differentiation·D LindholmL Korhonen
Jun 16, 2006·Journal of Neurotrauma·Stephen F LarnerKevin K W Wang
Jun 20, 2006·Current Opinion in Cell Biology·Lihong Zhao, Susan L Ackerman
Jul 6, 2006·Apoptosis : an International Journal on Programmed Cell Death·Daniela Sanges, Valeria Marigo
Aug 22, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Shanmugam Muruganandan, Alastair E Cribb
Feb 10, 2007·The FEBS Journal·Hiderou Yoshida
Apr 19, 2007·Journal of Neurotrauma·Jessie S TruettnerW Dalton Dietrich
Jun 23, 2007·Current Molecular Medicine·Hudson H Freeze
Sep 21, 2007·Biochemical and Biophysical Research Communications·Mi JangSung Goo Park
Aug 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Alexander H SteghRonald A DePinho
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·John G WalshSeamus J Martin
Dec 2, 2008·Nature Reviews. Drug Discovery·Inki KimJohn C Reed
Jun 12, 2009·The Journal of Biological Chemistry·Heidi K BaumgartnerOleg V Gerasimenko
Sep 23, 2009·Apoptosis : an International Journal on Programmed Cell Death·Zhiqun ZhangKevin K W Wang

❮ Previous
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Citations

Apr 22, 2011·Nature Reviews. Cancer·Sarah J StorrStewart G Martin
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Guixiang GanDixuan Jiang
Dec 18, 2013·International Journal of Molecular Medicine·Zhifang ZhaoHuimin Tao
Jul 17, 2012·Biomarker Insights·J Susie ZoltewiczLijuan Fang
Feb 25, 2014·Neurochemistry International·Qing XinBo Bai
Nov 5, 2013·Apoptosis : an International Journal on Programmed Cell Death·Selene García de la CadenaLourdes Massieu
Oct 5, 2011·Journal of Autism and Developmental Disorders·Dario SiniscalcoNicola Antonucci
Jul 12, 2011·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Wen-Chin WengHsinyu Lee
Dec 15, 2015·Biochimica Et Biophysica Acta·Charlotte DuboisFabien Vanden Abeele
Oct 7, 2015·Cell Biochemistry and Function·Areum Daseul KimJin Won Hyun
May 1, 2013·Journal of Cellular Physiology·Kayleen VannuvelThierry Arnould
Jun 15, 2014·Biochimica Et Biophysica Acta·Xiaoke ChiDavid W Andrews
Aug 31, 2013·Biochimica Et Biophysica Acta·Hery UrraClaudio Hetz
Nov 9, 2012·The International Journal of Neuroscience·Di LiuHongchao Yin
Jan 20, 2011·Expert Opinion on Therapeutic Targets·Ludovic Leloup, Alan Wells
Jan 13, 2015·Biochemical and Biophysical Research Communications·Wei XuNaihan Xu
Jul 30, 2014·Biochemical and Biophysical Research Communications·Yun-Rong ZhuYun-Qing Zhang
Apr 10, 2014·Molecular Neurobiology·Jie-Qiong LiLan Tan
May 5, 2016·Apoptosis : an International Journal on Programmed Cell Death·Selene García de la Cadena, Lourdes Massieu
May 8, 2016·Biochimica Et Biophysica Acta·Yuefang ZhouAlan Shiels
Sep 21, 2016·International Journal of Molecular Sciences·Entaz BaharHyonok Yoon
Oct 11, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lijuan ZhangPeng Fu
Nov 12, 2016·Cellular and Molecular Life Sciences : CMLS·Jeannette S Messer
Oct 28, 2016·Bioengineered·Genquan YinWenhui Jiang

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