Calpain Cleaves Most Components in the Multiple Aminoacyl-tRNA Synthetase Complex and Affects Their Functions.

The Journal of Biological Chemistry
Hui-Yan LeiEn-Duo Wang

Abstract

Nine aminoacyl-tRNA synthetases (aaRSs) and three scaffold proteins form a super multiple aminoacyl-tRNA synthetase complex (MSC) in the human cytoplasm. Domains that have been added progressively to MSC components during evolution are linked by unstructured flexible peptides, producing an elongated and multiarmed MSC structure that is easily attacked by proteases in vivo. A yeast two-hybrid screen for proteins interacting with LeuRS, a representative MSC member, identified calpain 2, a calcium-activated neutral cysteine protease. Calpain 2 and calpain 1 could partially hydrolyze most MSC components to generate specific fragments that resembled those reported previously. The cleavage sites of calpain in ArgRS, GlnRS, and p43 were precisely mapped. After cleavage, their N-terminal regions were removed. Sixty-three amino acid residues were removed from the N terminus of ArgRS to form ArgRSΔN63; GlnRS formed GlnRSΔN198, and p43 formed p43ΔN106. GlnRSΔN198 had a much weaker affinity for its substrates, tRNA(Gln) and glutamine. p43ΔN106 was the same as the previously reported p43-derived apoptosis-released factor. The formation of p43ΔN106 by calpain depended on Ca(2+) and could be specifically inhibited by calpeptin and by RNAi of ...Continue Reading

References

Apr 9, 2001·The Journal of Biological Chemistry·Y G KoS Kim
Apr 25, 2006·The Journal of Biological Chemistry·Yinfei TanPeter A Greer
Nov 15, 2006·The Journal of Cell Biology·Francesca DemarchiClaudio Schneider
Feb 27, 2007·Trends in Biochemical Sciences·Partho Sarothi RayPaul L Fox
Feb 5, 2008·Methods : a Companion to Methods in Enzymology·Sarah Ledoux, Olke C Uhlenbeck
Apr 9, 2008·Nature Chemical Biology·Andrea WilliamsDavid C Rubinsztein
Oct 29, 2008·The Journal of Biological Chemistry·Xiao-Long ZhouEn-Duo Wang
Jan 10, 2009·The Journal of Biological Chemistry·Monika KaminskaMarc Mirande
Sep 3, 2010·Nature Cell Biology·Noboru Mizushima, Beth Levine
Jun 15, 2011·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Hiroyuki SorimachiYasuko Ono
Sep 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Luisa BeltranPedro Rodriguez Cutillas
Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Peter S VoslerJun Chen
Nov 16, 2011·Autophagy·Jean-Paul DecuypereGeert Bultynck
Dec 20, 2011·Nucleic Acids Research·Thomas D GrantElizabeth J Grayhack
Feb 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Min Chul ParkSunghoon Kim
Mar 20, 2012·Molecular Cell·Grégory BonfilsClaudio De Virgilio
Feb 19, 2013·Nature Chemical Biology·Min Guo, Paul Schimmel
Mar 29, 2013·Topics in Current Chemistry·Peng YaoPaul L Fox
Mar 29, 2013·Topics in Current Chemistry·Min Guo, Xiang-Lei Yang
Sep 28, 2013·Topics in Current Chemistry·Jong Hyun KimSunghoon Kim
Jun 6, 2014·The Journal of Biological Chemistry·Fang YangEn-Duo Wang
Aug 26, 2014·Journal of Cell Science·Jong Hyun KimSunghoon Kim
Oct 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yaoyao FuYunje Cho
Dec 20, 2014·Cell Death and Differentiation·S ShaliniS Kumar
Dec 30, 2014·Ageing Research Reviews·Niki ChondrogianniEfstathios S Gonos
Feb 28, 2015·Nature Cell Biology·Diego L MedinaAndrea Ballabio

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Methods Mentioned

BETA
aminoacylation
two-hybrid
Co-immunoprecipitation
immunoprecipitation
co-IP
co-immunoprecipitate

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