Calpain contributes to silica-induced I kappa B-alpha degradation and nuclear factor-kappa B activation

Archives of Biochemistry and Biophysics
F ChenL M Demers

Abstract

Both silica and lipopolysaccharide (LPS) induce a rapid degradation of I kappa B alpha, an intracellular inhibitor of the nuclear factor (NF)-kappa B transcription factor. In this report, we demonstrate that MG132, a relatively specific proteasome inhibitor, is capable of suppressing LPS-induced I kappa B alpha degradation and NF-kappa B activation in mouse macrophage line RAW 264.7 cells, but is unable to influence the same induction produced by silica. In contrast, the lysosome inhibitor chloroquine has little effect on I kappa B alpha degradation induced by either silica or LPS. In fact, chloroquine enhances the signal-induced nuclear expression of NF-kappa B p50/p65 heterodimer by inhibiting the resynthesis of I kappa B alpha. With the use of transient transfection of a plasmid that expresses calpastatin, a natural inhibitor for calpain, the silica-induced degradation of I kappa B alpha and NF-kappa B activation was attenuated. In contrast, no inhibition of LPS-induced I kappa B alpha degradation and NF-kappa B activation was observed by the overexpression of calpastatin. This suggests that calpain contributes to silica-induced I kappa B alpha degradation and NF-kappa B activation but not to LPS-induced I kappa B alpha degr...Continue Reading

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Citations

Aug 7, 1998·Molecular Biology of the Cell·A M CuervoJ F Dice
Dec 19, 2000·Journal of Virology·R L DeBiasiK L Tyler
Nov 26, 2009·Yi chuan = Hereditas·Ju ZHANGHong-Bin LI
Mar 29, 2014·PLoS Computational Biology·Fabian KonrathDagmar Kulms
Apr 3, 2003·Biochemical and Biophysical Research Communications·Futoshi TeranishiTakashi Okamoto
Jul 28, 2016·Phytochemistry·Xing-De WuQin-Shi Zhao
Apr 23, 2010·Respirology : Official Journal of the Asian Pacific Society of Respirology·Michiyoshi NishiokaMichiaki Mishima
Oct 16, 1999·The Journal of Biological Chemistry·S D ShumwayS Miyamoto
Jul 22, 2005·The Journal of General Virology·Kasturi PalChristopher F Cuff
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Aug 16, 2000·American Journal of Physiology. Cell Physiology·F ChenX Shi
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Nov 24, 1999·Biochemical and Biophysical Research Communications·M S SeoS G Rhee
Jun 14, 2002·Biochimica Et Biophysica Acta·Tao LuRonald L Mellgren
Sep 24, 2008·Archives of Biochemistry and Biophysics·Roberto StifaneseEdon Melloni

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