Calpain inhibitor 2 prevents axonal degeneration of opossum optic nerve fibers

Journal of Neuroscience Research
Luciana Araújo CoutoAna Maria Blanco Martinez

Abstract

The ultrastructural change that characterizes the onset of Wallerian degeneration is the disintegration of axoplasmic microtubules and neurofilaments, which are converted into an amorphous and granular material, followed by myelin breakdown. The mechanism underlying such processes is an increase in the amount of intracellular calcium, leading to activation of proteases called calpains. The aim of this study was to evaluate by quantitative ultrastructural analysis whether nerve fibers can be preserved by the use of an exogenous inhibitor of these proteases (calpain inhibitor-2, Mu-F-hF-FMK), after optic nerve crush. For that, the left optic nerves of opossums, Didelphis aurita, were crushed with the aid of a fine forceps, and half of them received a calpain inhibitor mixed with Elvax resin. Ninety-six hours after the lesion, the animals were reanesthetized and transcardially perfused, and the optic nerves were removed, the right ones being used as normal nerves. Afterward, the optic nerves were dissected and processed for routine transmission electron microscopy and quantitative and statistical analysis. The results of this analysis showed that the group that received the calpain inhibitor presented a reduction of astrogliosis, ...Continue Reading

References

Apr 15, 1978·The Journal of Comparative Neurology·J N Hokoç, E O Cruz
Nov 15, 1991·Brain Research·M MataD J Fink
Jul 1, 1991·Physiological Reviews·D E Croall, G N DeMartino
Aug 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R L Mellgren
Aug 1, 1984·Archives of Biochemistry and Biophysics·G N Memartino, D E Croall
May 1, 1980·Journal of Neuropathology and Experimental Neurology·N L BanikE L Hogan
Jun 1, 1983·Experimental Neurology·B T StokesG Hollinden
May 4, 1981·Brain Research·R D HappelJ D Balentine
Jun 1, 1994·Journal of Neurochemistry·J D GlassJ W Griffin
Jan 15, 1993·Brain Research. Developmental Brain Research·A K ChakrabartiE L Hogan
Apr 1, 1997·Toxicology and Applied Pharmacology·R M LoPachin, E J Lehning
Nov 25, 1997·Annals of the New York Academy of Sciences·N L BanikE L Hogan
Jun 27, 1998·Biochemical and Biophysical Research Communications·E Carafoli, M Molinari
Sep 24, 1999·Journal of Neuroscience Research·S L Chan, M P Mattson
Feb 16, 2000·Progress in Retinal and Eye Research·M Dezawa, E Adachi-Usami
Dec 6, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A M Martinez, S Canavarro
Jun 26, 2001·Experimental Neurology·R W NeumarR Siman
Aug 23, 2001·Trends in Molecular Medicine·Y Huang, K K Wang
Nov 27, 2001·Neuroscience Letters·G Y HuhJ H Kim
Jul 23, 2002·Neuroscience Letters·Peter K Stys, Qiubo Jiang
Apr 5, 2003·Brain Research·Suelen Adriani MarquesAna Maria Blanco Martinez
May 10, 2003·Brain Research. Brain Research Reviews·Swapan K RayNaren L Banik
Jun 13, 2003·The Neurologist·Daniel BeckerJohn W McDonald
Sep 2, 2003·Seminars in Cell & Developmental Biology·Hideyuki OkanoYoshiaki Toyama

❮ Previous
Next ❯

Citations

Mar 21, 2006·Acta Neuropathologica·Maria Diaz-SanchezMargaret M Esiri
Feb 11, 2010·Journal of Neurotrauma·Rodrigo F Madeiro da CostaSergio T Ferreira
May 26, 2011·Journal of Neurotrauma·Fernanda Martins de AlmeidaAna Maria Blanco Martinez
Apr 2, 2013·Neurobiology of Disease·Marek MaRobert W Neumar
Nov 5, 2008·Molecular Neurobiology·Javier Sancho-PelluzFrançois Paquet-Durand
Apr 27, 2010·Journal of Neuropathology and Experimental Neurology·Bogdan BeirowskiMichael P Coleman
Apr 26, 2007·The American Journal of Pathology·Tanuja ChitnisSamia J Khoury
Dec 13, 2006·Experimental Eye Research·Levent SarikciogluArife Demirtop
Mar 16, 2007·Neuropathology and Applied Neurobiology·I M MedanaM M Esiri
Sep 3, 2010·Journal of Neurochemistry·François Paquet-DurandPer Ekström
Sep 26, 2006·Drug Discovery Today·Maria E SaezAgustin Ruiz
Dec 31, 2010·Journal of Neurotrauma·Ahmed Mohammed SulaimanWilliam L Maxwell
Feb 24, 2015·Frontiers in Cellular Neuroscience·Vanessa M MachadoInês M Araújo
Mar 24, 2016·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S de LimaP F Gardino
Dec 25, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Endre CzeiterJános Sándor
Dec 16, 2006·Journal of Neuroscience Research·François Paquet-DurandPer Ekström
Aug 10, 2005·Brain, Behavior and Evolution·Clynton Lourenço CorrêaAna Maria Blanco Martinez

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Kathryn E SaatmanT K McIntosh
© 2022 Meta ULC. All rights reserved