PMID: 9160241May 1, 1997Paper

Calpains and calpastatin in SH-SY5Y neuroblastoma cells during retinoic acid-induced differentiation and neurite outgrowth: comparison with the human brain calpain system

Journal of Neuroscience Research
F GrynspanR A Nixon

Abstract

Calpains have importance in human neurodegenerative disease pathogenesis, but these mechanisms are difficult to study in postmortem tissues. To establish a cellular model of the human calpain and calpastatin system, we characterized calpain I, calpain II, and calpastatin in SH-SY5Y human neuroblastoma cells in relation to their counterparts in human brain and investigated their expression and activity after inducing cellular differentiation with retinoic acid (RA), a physiological effector of normal brain development. Calpain I in both SH-SY5Y cells and human brain existed in the cytosolic and particulate fractions as three isoforms (80, 78, and 76 kDa) and exhibited atypical isoelectric focusing behavior. Calpain II in SH-SY5Y cells, as in human brain, migrated as a single predominantly cytosolic 76-kDa protein with an isoelectric point ranging from 5.9 to 6.3. Calpastatin from both sources was also 90% cytosolic. In the cells it was composed of four discrete bands, ranging in molecular weight from 110 to 127 kDa. Levels of activated (76 and 78 kDa) and precursor (80 kDa) calpain I isoforms rose 54% (P < 0.0001) in the particulate fraction and 26% (P < 0.0001) in the soluble fraction after 3 days of RA exposure. Because levels...Continue Reading

References

Aug 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D E GollT Zalewska
May 22, 1992·Biochimica Et Biophysica Acta·D E CroallC R Moomaw
Dec 18, 1991·European Journal of Biochemistry·M MichettiS Pontremoli
May 13, 1988·Science·J E Schollmeyer
Dec 30, 1988·Biochemical and Biophysical Research Communications·S PontremoliE Melloni
Feb 1, 1985·Journal of Neurochemistry·W W SchlaepferU J Zimmerman
Jul 31, 1986·Biochemical and Biophysical Research Communications·M InomataS Kawashima
Aug 1, 1984·Archives of Biochemistry and Biophysics·G N Memartino, D E Croall
Nov 1, 1983·Neurochemical Research·N L BanikM B Sostek
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R SimanG Lynch
Aug 16, 1984·Biochemical and Biophysical Research Communications·E TakanoT Murachi
Jan 1, 1981·Journal of Neurochemistry·B A BrownC A Marotta
Feb 1, 1995·The Journal of Experimental Zoology·N SargianosI Beis
Dec 15, 1994·Journal of Neuroscience Research·P LaengG Labourdette
Apr 18, 1994·FEBS Letters·H SorimachiK Suzuki

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Citations

Nov 21, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mala V RaoRalph A Nixon
Mar 26, 2010·The Journal of Biological Chemistry·Chunyang LiShi-Yong Sun
Jan 28, 2004·Annual Review of Pharmacology and Toxicology·Xiuli LiuRick G Schnellmann
Dec 13, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Matthew B Bevers, Robert W Neumar
Sep 6, 2003·Experimental Neurology·Manho KimMarian DiFiglia
Apr 30, 2005·Trends in Pharmacological Sciences·Harihara M Mehendale, Pallavi B Limaye
Mar 30, 2004·Journal of Neuroscience Research·Adrian T McCollumRodney P Guttmann
Jun 18, 2002·Journal of Neurochemistry·Jia HongMarsha Rich Rosner
Feb 16, 1999·Journal of Neurotrauma·J K NewcombR L Hayes
Oct 12, 2012·Journal of Molecular Modeling·Gaurav BhattiYeshitila Gebremichael
May 2, 2003·The Journal of Biological Chemistry·Mary SedarousDavid S Park

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