Calycosin-7-O-β-D-glucoside promotes oxidative stress-induced cytoskeleton reorganization through integrin-linked kinase signaling pathway in vascular endothelial cells

BMC Complementary and Alternative Medicine
Yue-hua JiangJing-Xin Xu

Abstract

Dysfunction of vascular endothelium is implicated in many pathological situations. Cytoskeleton plays an importance role in vascular endothelial permeability barrier and inflammatory response. Many Chinese herbs have the endothelial protective effect, of which, "Astragalus membranaceus" is a highly valued herb for treatment of cardiovascular and renal diseases in traditional Chinese medicine, In this study, we tested whether calycosin-7-O-β-D-glucoside (Calycosin), a main effective monomer component of "Astragalus membranaceus", could protect endothelial cells from bacterial endotoxin (LPS)-induced cell injury. Endothelial cell injury was induced by exposing human umbilical vein endothelial cells (HUVECs) to LPS. The effects of calycosin on LPS-induced changes in cell viability, apoptosis rate, cell migration, nitric oxide synthase (NOS), generationof intracellular reactive oxygen species (ROS) and cytoskeleton organization were determined. Microarray assay was employed to screen the possible gene expression change. Based on the results of microarray assay, the expression profile of genes involved in Rho/ROCK pathway and AKT pathway were further evaluated with quantitative real-time RT-PCR or western blot methods. Calycosin imp...Continue Reading

References

May 1, 1985·Cell·P Madaule, R Axel
Feb 27, 1999·Current Opinion in Cell Biology·G G Gundersen, T A Cook
Mar 10, 2001·Nature·L Chang, M Karin
May 16, 2002·The Journal of Biological Chemistry·Geraldine Pawlak, David M Helfman
Sep 19, 2002·Cardiovascular Research·Stefanie ZeukeHolger Heine
Feb 18, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Piero O BonettiAmir Lerman
May 18, 2005·The Journal of Biological Chemistry·Matvey GorovoyTatyana Voyno-Yasenetskaya
Sep 6, 2005·Developmental Cell·Atsushi EnomotoMasahide Takahashi
Dec 24, 2005·Physiological Reviews·Dolly Mehta, Asrar B Malik
Jun 27, 2007·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Nami OkamuraKunio Ishii
Feb 12, 2008·Nature Cell Biology·Tomoya KitamuraMasahide Takahashi
Feb 11, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Annunziata De LuisiAngelo Vacca
Aug 7, 2013·International Journal of Biological Macromolecules·Xiaofei WangJunhu Yao
Sep 17, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lee-Hsin ShawTung-Hu Tsai

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Citations

Jun 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nur Aqilah Binti KamaruddinYoke Keong Yong
Dec 21, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Hisa Hui Ling TsengMaggie Pui Man Hoi
Aug 11, 2018·BMC Complementary and Alternative Medicine·Hongbo LiChangjun Lv
May 27, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Huan LuXinbo Wu
Jan 20, 2021·Medicinal Research Reviews·Hui-Fei SuXue Qiao
Feb 25, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jie CuiJingcheng Dong

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Methods Mentioned

BETA
flow cytometry
chips
PCR
protein assay
electrophoresis
flowcytometry

Software Mentioned

Image J
GeneSpring GX

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