PMID: 9630712Jun 19, 1998Paper

Calyculin A modulates the kinetic constants for the Na+-coupled taurine transport in Ehrlich ascites tumour cells

Biochimica Et Biophysica Acta
J Mollerup, I H Lambert

Abstract

The effect of the phosphatase inhibitor calyculin A (cal A) on the kinetic parameters of the Na+-coupled taurine uptake via the taurine transporter in the Ehrlich ascites tumour cells has been investigated. Preincubation with cal A (100 nM) reduces the initial taurine influx by about 20%, but has no effect on the diffusional component of the taurine influx or on the taurine release from cells suspended in isotonic or in hypotonic medium. Thus, cal A-sensitive phosphatases only affect taurine transport mediated by the Na+-dependent taurine transporter. Cal A increases the Michaelis-Menten constant for binding of taurine to the transporter from 31+/-6 to 45+/-4 microM and reduces the taurine transport capacity from 210+/-20 to 170+/-10 nmol x g dry wt(-1) x min(-1) [corrected]. The Michaelis-Menten constant for binding of Na+ to the taurine transporter is concomitantly increased from 96+/-11 to 129+/-8 mM and the Na+:taurine coupling ratio for activation of the transport cycle is reduced from 3.3+/-0.6 to 2.4+/-0.2. This suggests that cal A-sensitive phosphatases maintain a high affinity of the taurine transporter towards Na+ and taurine as well as a high taurine transport capacity in unpertubated Ehrlich cells.

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Citations

Jun 11, 2008·Pflügers Archiv : European journal of physiology·Jack H JacobsenIan H Lambert
Aug 22, 2014·Acta Physiologica·I H LambertO H Mortensen
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ian Henry Lambert, Daniel Bloch Hansen
Sep 26, 2014·American Journal of Physiology. Cell Physiology·Belinda Halling SørensenIan Henry Lambert
Jun 3, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Renato Chaves Souto BrancoEverardo Magalhães Carneiro

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