Camel milk modulates the expression of aryl hydrocarbon receptor-regulated genes, Cyp1a1, Nqo1, and Gsta1, in murine hepatoma Hepa 1c1c7 cells.

Journal of Biomedicine & Biotechnology
Hesham M KorashyAyman O El-Kadi

Abstract

There is a traditional belief in the Middle East that camel milk may aid in prevention and treatment of numerous cases of cancer yet, the exact mechanism was not investigated. Therefore, we examined the ability of camel milk to modulate the expression of a well-known cancer-activating gene, Cytochrome P450 1a1 (Cyp1a1), and cancer-protective genes, NAD(P)H:quinone oxidoreductase 1 (Nqo1) and glutathione S-transferase a1 (Gsta1), in murine hepatoma Hepa 1c1c7 cell line. Our results showed that camel milk significantly inhibited the induction of Cyp1a1 gene expression by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the most potent Cyp1a1 inducer and known carcinogenic chemical, at mRNA, protein, and activity levels in a concentration-dependent manner. In addition, camel milk significantly decreased the xenobiotic responsive element (XRE)-dependent luciferase activity, suggesting a transcriptional mechanism is involved. Furthermore, this inhibitory effect of camel milk was associated with a proportional increase in heme oxygenase 1. On the other hand, camel milk significantly induced Nqo1 and Gsta1 mRNA expression level in a concentration-dependent fashion. The RNA synthesis inhibitor, actinomycin D, completely blocked the inductio...Continue Reading

References

May 1, 1992·The Journal of Dairy Research·E I el AgamyR Assaf
Apr 27, 1989·Nature·W R Brown
Oct 1, 1993·Analytical Biochemistry·A Lorenzen, S W Kennedy
May 20, 1999·Annual Review of Pharmacology and Toxicology·J P Whitlock
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 15, 2004·Cancer Science·Tsutomu Shimada, Yoshiaki Fujii-Kuriyama
Mar 19, 2004·The Journal of Biological Chemistry·Daniel W NebertFrank J Gonzalez
Jul 13, 2004·Cancer Treatment Reviews·S DansonM Ranson
Oct 22, 2004·American Journal of Epidemiology·Martha L SlatteryS Neuhausen
Nov 24, 2004·Drug Metabolism Reviews·David Ross
Aug 12, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hesham M Korashy, Ayman O S El-Kadi
Aug 24, 2005·Biochemical and Biophysical Research Communications·Goro KikuchiMasato Noguchi
Mar 28, 2006·Journal of Natural Products·Muriel CuendetJohn M Pezzuto
Apr 6, 2006·Human Genomics·Vasilis VasiliouDaniel W Nebert
Nov 1, 2007·The Journal of Dairy Research·Malik Sy HaddadinRichard K Robinson
Mar 11, 2008·Investigative Ophthalmology & Visual Science·Chandrassegar SaravananNoorjahan Panjwani
Apr 5, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Celia ConesaRobert W Evans
Aug 30, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Beshay N M ZordokyAyman O S El-Kadi
Mar 2, 2010·Free Radical Biology & Medicine·Anwar Anwar-Mohamed, Ayman O S El-Kadi
Mar 2, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Anca RoseanuMihaela Trif
Feb 2, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Anwar Anwar-MohamedAyman O S El-Kadi
Jun 15, 2011·Journal of Medicinal Food·Niki KontouAthena Linos

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Citations

Apr 3, 2013·Journal of Molecular Histology·Ziyu LiuZhikai Xu
Dec 11, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Abdulqader A AlhaiderSarita Saraswati
Jul 11, 2018·Integrative Cancer Therapies·Abdelnaser A BadawySana A AlSadrah
Sep 29, 2019·Clinical and Experimental Pharmacology & Physiology·Pritha BoseShakti P Pattanayak
Mar 7, 2019·Frontiers in Genetics·Amanat AliRanjit Vijayan
Oct 8, 2020·International Journal of Molecular Sciences·Ki-Uk KimHyeyoung Min
Jul 4, 2019·Journal of Agricultural and Food Chemistry·Emmagouni Sharath Kumar GoudSuneel Kumar Onteru

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BETA
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Software Mentioned

ImageJ
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