Camellia sinensis increased apoptosis on U2OS osteosarcoma cells and wound healing potential on NIH3T3 fibroblast cells

Cytotechnology
Sinem Er, Miriş Dikmen

Abstract

Camellia sinensis (Cs) is a plant which is rich in polyphenols and has antioxidant, antiinflammatory, antimutagenic, anticarcinogenic and antibacterial activities. In this study, two different methanol extracts (Cs-I and Cs-II) were prepared from the leaf of C. sinensis in order to investigate the wound healing and anticancer activities. Total phenolic content and antioxidant activity of the extracts were determined. Wound healing effects of Cs extracts were evaluated by using Masson's Trichrome Tecnique on NIH3T3 fibroblast cells. Cytotoxic and apoptotic effects of the extracts were determined by MTT and AnnexinV-PI assays on U2OS osteosarcoma cells. Total phenolic contents and antioxidant activities of the extracts were almost the same. The highest concentration (60 µg/mL) of the extracts showed significant cytotoxic and apoptotic effects on U2OS cells. Especially, the highest apoptotic effect was determined with 60 µg/mL Cs-I extract. Significant wound healing potential on NIH3T3 fibroblast cells were determined especially with low extract concentrations (0.5, 1 and 5 µg/mL), while high extract concentrations showed significant anticancer effects. As a result, two Cs leaf extracts exhibited important apoptotic properties and...Continue Reading

References

Mar 19, 1997·Cancer Letters·J H Weisburger
Oct 6, 1999·Biochemical Pharmacology·J K LinS Y Lin-Shiau
Aug 5, 2000·Archives of Dermatology·S K KatiyarH Mukhtar
Jan 24, 2002·Journal of Agricultural and Food Chemistry·S Khokhar, S G M Magnusdottir
Feb 18, 2003·Critical Reviews in Food Science and Nutrition·Jane V Higdon, Balz Frei
Mar 29, 2003·The Journal of Pharmacology and Experimental Therapeutics·Stephen HsuGeorge Schuster
Oct 7, 2004·European Journal of Pharmacology·Marcel W L Koo, Chi H Cho
Nov 13, 2004·The Journal of Pharmacology and Experimental Therapeutics·Stephen HsuGeorge Schuster
Dec 21, 2004·International Journal of Cancer. Journal International Du Cancer·Minakshi NihalGary S Wood
Jun 2, 2005·Journal of the American Academy of Dermatology·Stephen Hsu
Dec 13, 2006·Journal of Ethnopharmacology·Nilgün OztürkYusuf Oztürk
Sep 24, 2008·Plant Foods for Human Nutrition·Neda ShahsavariHasanali Naghdibadi
Jan 9, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·HakHee KimSuong-Hyu Hyon
Mar 28, 2009·International Journal of Molecular Sciences·Di Chen, Q P Dou
Feb 9, 2010·Journal of Dental Research·S Guo, L A Dipietro
Jan 6, 2011·Cancer Biotherapy & Radiopharmaceuticals·Miriş DikmenYagmur Tunali
Jul 19, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Fatemeh HajiaghaalipourJunedah Sanusi
Feb 18, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi-Shyan ChenChih-Chien Lin
Jan 1, 2000·Alternatives to Laboratory Animals : ATLA·S KorkmazH Can Baser
Oct 6, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Osman Tuncay AgarFatma Pinar Turkmenoglu

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Citations

Jul 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dan LiGang Wang
Oct 31, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Aleksandra ShedoevaChen Fan
Mar 27, 2020·ACS Applied Materials & Interfaces·Lili YaoKuang Sheng

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