CaMKIIalpha interacts with multi-PDZ domain protein MUPP1 in spermatozoa and prevents spontaneous acrosomal exocytosis

Journal of Cell Science
Frauke AckermannIngrid Boekhoff

Abstract

The success of acrosomal exocytosis, a complex process with a variety of inter-related steps, relies on the coordinated interaction of participating signaling molecules. Since the acrosome reaction resembles Ca(2+)-regulated exocytosis in neurons, we investigated whether cognate neuronal binding partners of the multi-PDZ domain protein MUPP1, which recruits molecules that control the initial tethering and/or docking between the acrosomal vesicle and the plasma membrane, are also expressed in spermatozoa, and whether they contribute to the regulation of acrosomal secretion. We observed that CaMKIIalpha colocalizes with MUPP1 in the acrosomal region of epididymal spermatozoa where the kinase selectively binds to a region encompassing PDZ domains 10-11 of MUPP1. Furthermore, we found that pre-treating mouse spermatozoa with a CaMKII inhibitor that directly blocks the catalytic region of the kinase, as well as a competitive displacement of CaMKIIalpha from PDZ domains 10-11, led to a significant increase in spontaneous acrosomal exocytosis. Since Ca(2+)-calmodulin releases CaMKIIalpha from the PDZ scaffolding protein, MUPP1 represents a central signaling platform to dynamically regulate the assembly and disassembly of binding partn...Continue Reading

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Citations

Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Matías A BustosClaudia N Tomes
Jun 17, 2010·The Journal of Biological Chemistry·Jimena Castillo BennettLuis S Mayorga
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Oct 25, 2020·International Journal of Molecular Sciences·Deborah Allouche-Fitoussi, Haim Breitbart
Apr 26, 2020·Theriogenology·Y HutaH Breitbart
Jul 28, 2020·Molecular and Cellular Endocrinology·Maya FinkelsteinHaim Breitbart
Sep 8, 2011·Journal of Cell Science·Christina Hornbruch-FreitagRenate Renkawitz-Pohl
Mar 22, 2014·Journal of Cell Science·Sabrina BaumgartEva M Neuhaus

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