cAMP and Ca2+ involvement in the mitochondrial response of cultured fetal rat hepatocytes to adrenaline

Experimental Cell Research
M V GarcíaJ M Medina

Abstract

The effect of adrenaline on the control of respiratory activity of mitochondria from fetal hepatocytes in primary culture was studied. In the absence of adrenaline, the respiratory control ratio (RCR) of mitochondria increased during the first 3 days of culture due to a decrease in the rate of state 4 respiration. The presence of adrenaline in the incubation medium further increased the mitochondrial RCR through a decrease in the rate of respiration in state 4 and to an increase in the respiration rate in state 3. The effect of adrenaline was mimicked by dibutyryl-cAMP, forskolin, and isobutyl methyl xanthine. All these compounds increased cAMP concentrations, suggesting that cAMP may be involved in the effect of adrenaline. The increase in intracellular free Ca2+ concentrations caused by phenylephrine, vasopressin, or thapsigargin was also accompanied by an increase in the RCR, suggesting that both phenomena are associated. Dibutyryl-cAMP also increased free Ca2+ concentrations, suggesting that the effects of cAMP may be mediated by free Ca2+ concentrations. Adrenaline, dibutyryl-cAMP, phenylephrine, vasopressin, and thapsigargin promoted adenine nucleotide accumulation in mitochondria; this may be an intermediate step in the ...Continue Reading

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Citations

Mar 29, 2003·Biochemical Pharmacology·Gabor VarbiroFerenc Gallyas
Jan 24, 2007·Physiological Reviews·Jorgina SatrústeguiAraceli Del Arco
Sep 13, 2003·The Journal of Pharmacology and Experimental Therapeutics·Gabor VarbiroFerenc Gallyas
Nov 9, 2019·Seminars in Cell & Developmental Biology·Saharnaz SarlakRodrigue Rossignol
Feb 17, 2001·General and Comparative Endocrinology·G KrumschnabelP J Schwarzbaum
Aug 21, 2013·Bioorganic & Medicinal Chemistry Letters·Yuri K PetersonRick G Schnellmann

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