cAMP counter-regulates insulin-mediated protein phosphatase-2A inactivation in rat skeletal muscle cells.

The Journal of Biological Chemistry
N Begum, L Ragolia

Abstract

In this study, we examined the mechanism of recently reported inactivation of protein phosphatase-2A (PP-2A) by insulin (Srinivasan, M., and Begum, N. (1994) J. Biol. Chem. 269, 12514-12520) and its counter-regulation by cAMP agonists. Exposure of L6 myotubes to insulin resulted in a rapid inhibition of PP-2A that was accompanied by a 3-fold increase in the phosphotyrosine content of the immunoprecipitated PP-2A catalytic subunit. Pretreatment with (Sp)-cAMP, a cAMP agonist, completely blocked insulin-mediated inhibition of PP-2A activity and decreased the tyrosine phosphorylation of PP-2A catalytic subunit to control levels. To understand the mechanism of counter-regulation of PP-2A by (Sp)-cAMP, cells were pretreated with sodium orthovanadate, an inhibitor of phosphotyrosine phosphatases. Vanadate prevented the effect of (Sp)-cAMP on PP-2A activity and increased the phosphorylation status of PP-2A catalytic subunit to the level observed with insulin. Wortmannin, a phosphatidylinositol 3-kinase inhibitor, and rapamycin, an inhibitor of 70-kDa S6 kinase activation, prevented insulin-mediated inactivation of PP-2A, suggesting that these pathways may participate in insulin-mediated phosphorylation and inactivation of PP-2A. These...Continue Reading

References

Aug 1, 1991·Molecular and Cellular Biology·R RuedigerG Walter
Jan 1, 1989·Annual Review of Biochemistry·P Cohen
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jan 1, 1983·Methods in Enzymology·P Cohen
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H Guo, Z Damuni
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L M GravesE G Krebs
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B R SevetsonJ C Lawrence
Nov 12, 1993·Science·S J Cook, F McCormick
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·F T StevensonD H Lovett
Aug 1, 1994·Trends in Cell Biology·R E Mayer-Jaekel, B A Hemmings

❮ Previous
Next ❯

Citations

Apr 12, 2002·Journal of Cellular Biochemistry·David Hartley, Geoffrey M Cooper
May 6, 2011·Calcified Tissue International·Aki KashiwagiMasako Shimada
Mar 31, 1998·Biochimica Et Biophysica Acta·A Gómez-Muñoz
Jul 22, 1999·Diabetes Research and Clinical Practice·K KanekoM Shichiri
Jan 14, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M N PertsevaL A Kuznetsova
Aug 3, 2006·Nature Reviews. Drug Discovery·Sandrine FaivreEric Raymond
Aug 31, 2006·Anti-cancer Drugs·Hong-bo ShanLi-jian Xian
Dec 12, 2003·Science's STKE : Signal Transduction Knowledge Environment·Thurl E Harris, John C Lawrence
Jan 20, 2004·Molecular and Cellular Biology·Lazaros C FoukasPeter R Shepherd
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Ken InokiKun-Liang Guan
May 21, 2010·Molecular Cancer·Ali NaderiLuke Hughes-Davies
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·A NishiP Greengard
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·R T PetersonS L Schreiber
Mar 11, 1999·Current Opinion in Genetics & Development·P B DennisG Thomas
Mar 28, 2008·Journal of Cellular and Molecular Medicine·J Avila
Aug 28, 2009·International Journal of Cancer. Journal International Du Cancer·Ali NaderiIan C Bennett
Oct 29, 2015·The FEBS Journal·Jaya SangodkarGoutham Narla
Nov 6, 2015·Mediators of Inflammation·Chong-xin HuangYue Wang
Jun 23, 2006·Pharmacology & Therapeutics·Alistair T R SimNicole M Verrills
Dec 2, 1999·Experimental Cell Research·A Dufner, G Thomas
Mar 17, 2015·Brain : a Journal of Neurology·Sandy R ShultzTerence J O'Brien
Aug 16, 2016·Molecular and Cellular Endocrinology·Muheeb BegAnil N Gaikwad
Feb 12, 1998·The Journal of Biological Chemistry·M AndjelkovićB A Hemmings
Aug 3, 2004·Microscopy Research and Technique·Othon B KotoulasDimitrios J Kondomerkos
Jan 22, 2005·The Journal of Biological Chemistry·Limin MaoJohn Q Wang
Jun 18, 2002·The Journal of Pharmacology and Experimental Therapeutics·Marina S FeschenkoKathleen J Sweadner
Aug 15, 2000·The Journal of Biological Chemistry·M S FeschenkoK J Sweadner
Apr 17, 2003·The Journal of Biological Chemistry·Christine E PullarR Rivkah Isseroff

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.