cAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells

Scientific Reports
Carolina IndaSusana Silberstein

Abstract

Corticotropin-releasing hormone receptor 1 (CRHR1) activates the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). Both cAMP sources were shown to be required for the phosphorylation of ERK1/2 triggered by activated G protein coupled receptor (GPCR) CRHR1 in neuronal and neuroendocrine contexts. Here, we show that activated CRHR1 promotes growth arrest and neurite elongation in neuronal hippocampal cells (HT22-CRHR1 cells). By characterising CRHR1 signalling mechanisms involved in the neuritogenic effect, we demonstrate that neurite outgrowth in HT22-CRHR1 cells takes place by a sAC-dependent, ERK1/2-independent signalling cascade. Both tmACs and sAC are involved in corticotropin-releasing hormone (CRH)-mediated CREB phosphorylation and c-fos induction, but only sAC-generated cAMP pools are critical for the neuritogenic effect of CRH, further highlighting the engagement of two sources of cAMP downstream of the activation of a GPCR, and reinforcing the notion that restricted cAMP microdomains may regulate independent cellular processes.

References

Jan 7, 1972·Science·F J RoisenW G Braden
Nov 1, 1994·In Vitro Cellular & Developmental Biology. Animal·M F Melzig
Apr 1, 1996·Molecular and Cellular Biology·W L KuoM R Rosner
Nov 26, 1999·The Journal of Investigative Dermatology·J L ArbiserJ A Majzoub
Jun 21, 2002·Trends in Cell Biology·Philip J S Stork, John M Schmitt
Mar 1, 2003·The Journal of Biological Chemistry·Tatiana N LitvinLonny R Levin
Apr 5, 2003·Annual Review of Biochemistry·Eric J Huang, Louis F Reichardt
Jun 5, 2003·Molecular Neurobiology·Kristen L BrunsonTallie Z Baram
Aug 28, 2003·The Biochemical Journal·Dermot M F Cooper
Sep 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bijay S Jaiswal, Marco Conti
Oct 15, 2003·Neuron·George J AugustineKeiko Tanaka
Aug 3, 2004·Nature Neuroscience·Jens C Schwamborn, Andreas W Püschel
Oct 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yuncai ChenTallie Z Baram
Dec 13, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jacek JaworskiMorgan Sheng
Apr 22, 2006·The Journal of Biological Chemistry·Alexander M StessinLonny R Levin
May 25, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Andrzej SlominskiDesmond J Tobin
Aug 22, 2006·CNS & Neurological Disorders Drug Targets·Richard L HaugerFrank M Dautzenberg
Sep 12, 2006·Nature Neuroscience·Karen Y WuSamie R Jaffrey
Apr 5, 2007·Nature Protocols·Min Jiang, Gong Chen
Aug 8, 2007·Journal of Neuroscience Research·Jennifer J YoungAlexander M Stessin
Feb 15, 2008·European Journal of Pharmacology·Florian Holsboer, Marcus Ising
Apr 11, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Simon W MooreTimothy E Kennedy
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yuncai ChenTallie Z Baram
Nov 9, 2010·Journal of Proteomics·Juan J BonfiglioSusana Silberstein
Mar 10, 2011·Nature Reviews. Neuroscience·Takuro TojimaHiroyuki Kamiguchi
Apr 15, 2011·Kidney International·Martin TresguerresJochen Buck
Dec 31, 2011·Molecular Cell·Jia-Yun ChenTobias Meyer
Apr 26, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrew C Emery, Lee E Eiden

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Citations

Aug 16, 2018·Physiological Reviews·Jan M Deussing, Alon Chen
May 5, 2018·Biomedical Reports·Kosuke HamajimaAkifumi Togari
Jul 23, 2020·Trends in psychiatry and psychotherapy·Mailton VasconcelosRosa Maria M de Almeida
May 13, 2018·Journal of Cardiovascular Development and Disease·Sofya Pozdniakova, Yury Ladilov
Dec 8, 2020·Frontiers in Molecular Neuroscience·José Ávila-MendozaRobert J Denver

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Methods Mentioned

BETA
PCR
FRET
biosensor
flow cytometry
biosensors
transfection

Software Mentioned

GraphPad Prism
ZEN Black
Fluoview
Zeiss Zen Blue 2011
Zen Blue 2011
Fiji
Metamorph
ZEISS

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