cAMP-dependent protein kinase A in grass carp Ctenopharyngodon idella: Molecular characterization, gene structure, tissue distribution and mRNA expression in endoplasmic reticulum stress-induced adipocyte lipolysis.

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
ShangHong JiHong Ji

Abstract

Protein kinase A (PKA), one of the most widely studied protein kinases, has many functions in cells, including regulating the metabolism of sugar and lipid. Here we identified nine isoforms of the PKA family in grass carp Ctenopharyngodon idella and obtained their complete coding sequences (CDS), including PRKACAa, PRKACAb, PRKACBa, PRKACBb, PRKAR1A, PRKAR1B, PRKAR2Aa, PRKAR2Ab and PRKAR2B, and PRKACA, PRKACB and PRKAR2A, which may experience fish-specific genome duplication. Sequence analysis showed that the predicted protein structures of PKA gene family members in grass carp were different. Grass carp PRKACAa, PRKACAb, PRKACBa, and PRKACBb contained serine/threonine protein kinases, while PRKAR1A, PRKAR1B, PRKAR2Aa, PRKAR2Ab and PRKAR2B contained two cyclic nucleotide-monophosphate binding domains. PRKACAa, PRKACBa, PRKACBb, PRKAR1A, PRKAR1B and PRKAR2Aa contained 10 coding exons, while PRKACAb and PRKAR2Ab consisted of 12 coding exons and 5 coding exons, respectively. The messenger RNA (mRNA) of the nine PKA isoforms was detected in a wide range of tissues, but their abundance showed tissue-dependent expression patterns. In tunicamycin-induced adipocyte lipolysis, only the mRNA levels of PRKACAb and PRKACBa showed a signifi...Continue Reading

References

Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J D ScottE G Krebs
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M D UhlerG S McKnight
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D C LeeG S McKnight
Feb 14, 1997·The Journal of Biological Chemistry·P S AmieuxG S McKnight
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·J L DesseynG S McKnight
Jun 8, 2000·Annals of the New York Academy of Sciences·C LondosC M Rondinone
Jul 18, 2002·Annals of the New York Academy of Sciences·Paul S Amieux, G Stanley McKnight
Oct 9, 2002·Trends in Biochemical Sciences·Gerard ManningSucha Sudarsanam
Oct 9, 2002·Genome Research·Lewis Y GeerStephen H Bryant
May 13, 2003·The Journal of Biological Chemistry·Turid EidePhilippe Collas
Jun 18, 2003·The Journal of Cell Biology·Carole SztalrydConstantine Londos
Oct 16, 2004·Science·Umut OzcanGökhan S Hotamisligil
Nov 20, 2004·Science·Robert ZimmermannRudolf Zechner
Jun 15, 2005·Annual Review of Biochemistry·Martin Schröder, Randal J Kaufman
Jun 28, 2005·Nucleic Acids Research·E QuevillonR Lopez
Nov 1, 2006·The Journal of Biological Chemistry·Martina SchweigerRobert Zimmermann
Mar 1, 2007·American Journal of Physiology. Endocrinology and Metabolism·Mikael RydénPeter Arner
Feb 12, 2009·American Journal of Physiology. Endocrinology and Metabolism·Qiu Gen ZhouXin Peng
Jan 10, 2012·The Journal of Biological Chemistry·Jingna DengGuoheng Xu
Jan 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Guixia XuHongzhi Kong
Mar 9, 2012·Biochemistry Research International·Beth S Zha, Huiping Zhou
Mar 13, 2012·Cell Metabolism·Rudolf ZechnerFrank Madeo
Jul 10, 2012·Trends in Endocrinology and Metabolism : TEM·Mélissa FlammentFabienne Foufelle
Aug 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Thomas J NearW Leo Smith
Apr 18, 2013·PloS One·Kristoffer SøbergJon K Laerdahl
Jan 21, 2014·Cell·Evan D Rosen, Bruce M Spiegelman
Nov 11, 2014·Journal of Cellular and Molecular Medicine·Elena BogdanovicMarc G Jeschke

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Genetics in Medicine : Official Journal of the American College of Medical Genetics
Ludivine DrougatConstantine A Stratakis
Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology
Nicki Yat Hin LeungTing Fan Leung
© 2021 Meta ULC. All rights reserved