Abstract
Salt-inducible kinase (SIK) 3 is a virtually unstudied, ubiquitously expressed serine/threonine kinase, belonging to the AMP-activated protein kinase (AMPK)-related family of kinases, all of which are regulated by LKB1 phosphorylation of a threonine residue in their activation (T)-loops. Findings in adrenal cells have revealed a role for cAMP in the regulation of SIK1, and recent findings suggest that insulin can regulate an SIK isoform in Drosophila. As cAMP has important functions in adipocytes, mainly in the regulation of lipolysis, we have evaluated a potential role for cAMP, as well as for insulin, in the regulation of SIK3 in these cells. We establish that raised cAMP levels in response to forskolin and the β-adrenergic receptor agonist CL 316,243 induce a phosphorylation of SIK3 in HEK293 cells and primary adipocytes. This phosphorylation coincides with increased 14-3-3 binding to SIK3 in these cell types. Our findings also show that cAMP-elevation results in reduced SIK3 activity in adipocytes. Phosphopeptide mapping and site-directed mutagenesis reveal that the cAMP-mediated regulation of SIK3 appears to depend on three residues, T469, S551 and S674, that all contribute to some extent to the cAMP-induced phosphorylatio...Continue Reading
References
Sep 1, 1996·Current Biology : CB·G MoorheadC MacKintosh
Aug 30, 2002·The Journal of Biological Chemistry·Hiroshi TakemoriMitsuhiro Okamoto
Aug 28, 2003·The Journal of Biological Chemistry·Wu YinMorris J Birnbaum
Nov 15, 2003·Current Biology : CB·Angela WoodsDavid Carling
Dec 25, 2003·Trends in Endocrinology and Metabolism : TEM·Mitsuhiro OkamotoYoshiko Katoh
Feb 21, 2004·The EMBO Journal·Jose M LizcanoDario R Alessi
Feb 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Reuben J ShawLewis C Cantley
May 29, 2004·The Biochemical Journal·Carol Mackintosh
Sep 30, 2004·Cell·Robert A ScreatonMarc Montminy
Dec 24, 2004·Nature·M Bettencourt-DiasD M Glover
Jun 18, 2005·Cellular Signalling·Emilia Zmuda-TrzebiatowskaEva Degerman
Sep 15, 2005·Diabetologia·D S HutchinsonT Bengtsson
Nov 25, 2005·Journal of Cell Science·Abdallah K Al-HakimDario R Alessi
Jul 15, 2006·Cellular Signalling·Emilia Zmuda-TrzebiatowskaEva Degerman
Sep 14, 2007·The Biochemical Journal·Jenny BainPhilip Cohen
Jan 27, 2009·Cellular Signalling·Bilal OmarEva Degerman
Apr 1, 2010·Science Signaling·Anna ZagórskaDario R Alessi
Mar 15, 2011·Oncogene·S CharoenfuprasertN-Y Shih
May 14, 2011·Cell·Biao WangMarc Montminy
Feb 10, 2012·Development·Satoru SasagawaNoriyuki Tsumaki
Citations
Dec 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Randi StewartRebecca Berdeaux
May 27, 2014·Journal of Molecular Endocrinology·Amélie GormandOlga Göransson
Mar 25, 2016·Nature Communications·Yasuhito YaharaNoriyuki Tsumaki
Nov 4, 2016·Diabetologia·Johanna SällOlga Göransson
Jun 29, 2017·PloS One·Suneetha AmaraVenkataswarup Tiriveedhi
Nov 5, 2016·Nature·Hiromasa FunatoMasashi Yanagisawa
Dec 7, 2017·The FEBS Journal·Tim SonntagMarc Montminy
Aug 21, 2019·The Journal of Clinical Investigation·Shigeki NishimoriMarc N Wein
Sep 27, 2018·Proceedings of the National Academy of Sciences of the United States of America·Takato HondaMasashi Yanagisawa
Jun 17, 2020·PloS One·H Bahar ŞahinArzu Çelik
Mar 30, 2019·PeerJ·Meiling HongLi Ding
May 28, 2020·Scientific Reports·Minjeong ParkHiromasa Funato
Feb 25, 2017·PloS One·Tim SonntagMarc Montminy
Mar 2, 2021·ELife·Hailun LiAaron DiAntonio
Apr 17, 2021·The Biochemical Journal·Nicola J Darling, Philip Cohen
Dec 5, 2014·Journal of Cell Science·Emma HenrikssonOlga Göransson
Jun 24, 2021·ELife·Cheng-Chia TangMarc N Wein
Sep 21, 2018·Science Translational Medicine·Fabiana CsukasiDeborah Krakow