cAMP-elevation mediated by β-adrenergic stimulation inhibits salt-inducible kinase (SIK) 3 activity in adipocytes.

Cellular Signalling
Christine BerggreenOlga Göransson

Abstract

Salt-inducible kinase (SIK) 3 is a virtually unstudied, ubiquitously expressed serine/threonine kinase, belonging to the AMP-activated protein kinase (AMPK)-related family of kinases, all of which are regulated by LKB1 phosphorylation of a threonine residue in their activation (T)-loops. Findings in adrenal cells have revealed a role for cAMP in the regulation of SIK1, and recent findings suggest that insulin can regulate an SIK isoform in Drosophila. As cAMP has important functions in adipocytes, mainly in the regulation of lipolysis, we have evaluated a potential role for cAMP, as well as for insulin, in the regulation of SIK3 in these cells. We establish that raised cAMP levels in response to forskolin and the β-adrenergic receptor agonist CL 316,243 induce a phosphorylation of SIK3 in HEK293 cells and primary adipocytes. This phosphorylation coincides with increased 14-3-3 binding to SIK3 in these cell types. Our findings also show that cAMP-elevation results in reduced SIK3 activity in adipocytes. Phosphopeptide mapping and site-directed mutagenesis reveal that the cAMP-mediated regulation of SIK3 appears to depend on three residues, T469, S551 and S674, that all contribute to some extent to the cAMP-induced phosphorylatio...Continue Reading

References

Aug 28, 2003·The Journal of Biological Chemistry·Wu YinMorris J Birnbaum
Nov 15, 2003·Current Biology : CB·Angela WoodsDavid Carling
Dec 25, 2003·Trends in Endocrinology and Metabolism : TEM·Mitsuhiro OkamotoYoshiko Katoh
Feb 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Reuben J ShawLewis C Cantley
Dec 24, 2004·Nature·M Bettencourt-DiasD M Glover
Nov 25, 2005·Journal of Cell Science·Abdallah K Al-HakimDario R Alessi
Sep 14, 2007·The Biochemical Journal·Jenny BainPhilip Cohen
May 14, 2011·Cell·Biao WangMarc Montminy
Feb 10, 2012·Development·Satoru SasagawaNoriyuki Tsumaki

❮ Previous
Next ❯

Citations

Dec 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Randi StewartRebecca Berdeaux
May 27, 2014·Journal of Molecular Endocrinology·Amélie GormandOlga Göransson
Mar 25, 2016·Nature Communications·Yasuhito YaharaNoriyuki Tsumaki
Nov 5, 2016·Nature·Hiromasa FunatoMasashi Yanagisawa
Aug 21, 2019·The Journal of Clinical Investigation·Shigeki NishimoriMarc N Wein
Sep 27, 2018·Proceedings of the National Academy of Sciences of the United States of America·Takato HondaMasashi Yanagisawa
May 28, 2020·Scientific Reports·Minjeong ParkHiromasa Funato
Apr 17, 2021·The Biochemical Journal·Nicola J Darling, Philip Cohen
Dec 5, 2014·Journal of Cell Science·Emma HenrikssonOlga Göransson
Sep 21, 2018·Science Translational Medicine·Fabiana CsukasiDeborah Krakow

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.