PMID: 9447925Feb 3, 1998Paper

cAMP increases K+ secretion via activation of apical IsK/KvLQT1 channels in strial marginal cells

Hearing Research
H SunoseDaniel C Marcus

Abstract

In the cochlea, K+ is secreted by electrodiffusion across the apical membrane of strial marginal cells via the IsK/KvLQT1 ('IsK') channel. This channel complex has been reported to be activated in other systems by adenosine 3',5'-cyclic monophosphate (cAMP). Since several reports had suggested that cAMP is a second messenger in the cochlea, the effect of the cAMP pathway on transepithelial K+ secretion by strial marginal cells of the gerbil was studied. Both the transepithelial current (Isc) and K+ flux (JK) across strial marginal cell epithelium were measured; Isc in a micro-Ussing chamber and JK as the gradient of K+ concentration near the apical membrane. The apical membrane current (IIsK) and conductance (gIsK) of IsK channels were recorded with the on-cell macro-patch and the nystatin-perforated whole-cell patch clamp techniques. It has previously been shown that the apical IsK channel constitutes the primary pathway for K+ secretion. Cytoplasmic cAMP was elevated by applying dibutyryl cyclic-AMP (dbcAMP) or the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) at 37 degrees C. dbcAMP (1 mM) increased Isc by 51 +/- 4% and IIsK in on-cell and whole-cell recordings increased by 214 +/- 63% and 390 +/- 61% above ...Continue Reading

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Citations

Apr 14, 2000·Brain Research. Molecular Brain Research·M J DrescherD G Drescher
Oct 12, 2001·Brain Research Bulletin·B Fritzsch, K W Beisel
Aug 17, 2001·Hearing Research·J H Lee, D C Marcus
May 29, 2002·Hearing Research·Philine Wangemann
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Sep 26, 2015·American Journal of Physiology. Cell Physiology·Martin N AndersenHanne B Rasmussen

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