cAMP regulates the functional activity, coupling efficiency and structural organization of mammalian FOF1 ATP synthase

Biochimica Et Biophysica Acta
Domenico De RasmoS Papa

Abstract

The present study shows that in isolated mitochondria and myoblast cultures depletion of cAMP, induced by sAC inhibition, depresses both ATP synthesis and hydrolysis by the FOF1 ATP synthase (complex V) of the oxidative phosphorylation system (OXPHOS). These effects are accompanied by the decrease of the respiratory membrane potential, decreased level of FOF1 connecting subunits and depressed oligomerization of the complex. All these effects of sAC inhibition are prevented by the addition of the membrane-permeant 8-Br-cAMP. These results show, for the first time, that cAMP promotes ATP production by complex V and prevents, at the same time, its detour to a mitochondrial membrane leak conductance, which is involved in cell death.

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Citations

May 29, 2016·Cellular and Molecular Life Sciences : CMLS·Fan ZhangHong Xu
Apr 27, 2019·CNS Neuroscience & Therapeutics·Maribel LuceroJeremy W Chambers
Apr 5, 2020·International Journal of Molecular Sciences·Ines AmaraVittorio Calabrese
Mar 20, 2018·Cellular and Molecular Life Sciences : CMLS·Francesco BellomoDomenico De Rasmo
May 13, 2018·Journal of Cardiovascular Development and Disease·Sofya Pozdniakova, Yury Ladilov
Feb 13, 2021·Antioxidants·Petr JežekLydie Plecitá-Hlavatá
Aug 24, 2020·Biochemical and Biophysical Research Communications·Ljudmila S KhailovaYuri N Antonenko
Jun 29, 2021·Antioxidants & Redox Signaling·Petr JežekLydie Plecitá-Hlavatá

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