cAmp regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation

The Journal of Biological Chemistry
S L CoonD C Klein

Abstract

Arylalkylamine N-acetyltransferase (serotonin N-acetyltransferase, AANAT, EC ) is the penultimate enzyme in melatonin synthesis. As described here, a cell line (1E7) expressing human AANAT (hAANAT) has been developed to study the human enzyme. 1E7 hAANAT is detectable in immunoblots as a 23-kDa band and is immunocytochemically visualized in the cytoplasm. The specific concentration of hAANAT in homogenates is comparable to that of the night rat pineal gland. Kinetics of AANAT extracted from 1E7 cells are the same as those of bacterially expressed hAANAT; both preparations of hAANAT are equally sensitive to the inhibitor CoA-S-N-acetyltryptamine. Studies of cAMP regulation indicate that treatment with forskolin, dibutyryl cAMP, isobutylmethylxanthine, or isoproterenol activate cellular hAANAT within intact 1E7 cells approximately 8-fold without markedly increasing the abundance of AANAT protein or the activity of AANAT in broken cell preparations; and, that forskolin, isobutylmethylxanthine and isoproterenol elevate cyclic AMP production. These observations extend our understanding of cAMP regulation of AANAT activity, because it is currently thought that this only involves changes in the steady-state levels of AANAT protein. Th...Continue Reading

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Citations

Aug 6, 2003·European Journal of Biochemistry·Andrzej SlominskiJacobo Wortsman
Jan 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Surajit GangulyDavid C Klein
Oct 31, 2002·The Journal of Investigative Dermatology·Andrzej SlominskiJacobo Wortsman
Dec 9, 2014·PloS One·Damien Cazaméa-CatalanElodie Magnanou
Sep 7, 2002·The Journal of Biological Chemistry·Seiji TsuboiYoshinori Moriyama
Jan 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrzej SlominskiDesmond J Tobin
Mar 4, 2008·Journal of Pineal Research·Dave LanoixCathy Vaillancourt
Jul 5, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ying-Hui WuDick F Swaab
Dec 14, 2006·The Journal of Biological Chemistry·David C Klein
Sep 10, 2008·Chronobiology International·Khalid El AllaliValérie Simonneaux
Oct 5, 2002·The Journal of Clinical Endocrinology and Metabolism·Steven L CoonDavid C Klein
Dec 29, 2020·PLoS Computational Biology·Bojan KrtenicNathalie Reuter

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