cAMP-response element-binding protein mediates tumor necrosis factor-alpha-induced vascular smooth muscle cell migration

Arteriosclerosis, Thrombosis, and Vascular Biology
Hiroki OnoAkira Takeshita

Abstract

Migration of vascular smooth muscle cells (VSMCs) contributes to formation of vascular stenotic lesions such as atherosclerosis and restenosis after angioplasty. Previous studies have demonstrated that tumor necrosis factor-alpha (TNF-alpha) is a potent migration factor for VSMCs. cAMP-response element-binding protein (CREB) is the stimulus-induced transcription factor and activates transcription of target genes such as c-fos and interleukin-6. We examined whether CREB is involved in TNF-alpha-induced VSMC migration. TNF-alpha induced CREB phosphorylation with a peak at 15 minutes of stimulation. Pharmacological inhibition of p38 mitogen-activated protein kinase (p38-MAPK) inhibited TNF-alpha-induced CREB phosphorylation. Adenovirus-mediated overexpression of dominant-negative form of CREB suppressed TNF-alpha-induced CREB phosphorylation and c-fos mRNA expression. VSMC migration was evaluated using a Boyden chamber. Overexpression of dominant-negative form of CREB suppressed VSMC migration as well as Rac1 expression induced by TNF-alpha. Overexpression of dominant-negative Rac1 also inhibited TNF-alpha-induced VSMC migration. Our results suggest that p38-MAPK/CREB/Rac1 pathway plays a critical role in TNF-alpha-induced VSMC mi...Continue Reading

References

Aug 1, 1990·Genes & Development·F WeihG Schütz
Nov 24, 1995·Science·Z XiaM E Greenberg
Aug 11, 1994·Nucleic Acids Research·M G Anderson, W S Dynan
Oct 1, 1995·Cardiovascular Research·H Abedi, I Zachary
May 1, 1996·The Journal of Cell Biology·A M Murphy, D J Montell
Feb 7, 1998·Science·A Hall
Nov 26, 1998·The Journal of Biological Chemistry·K Du, M Montminy
Mar 24, 1999·The Journal of Cell Biology·C D Nobes, A Hall
Aug 14, 1999·The Journal of Biological Chemistry·J C HedgesW T Gerthoffer
Dec 28, 1999·Science·A R Horwitz, J T Parsons
Jun 29, 2000·Annual Review of Biochemistry·A J Shaywitz, M E Greenberg
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·B Mayr, M Montminy

❮ Previous
Next ❯

Citations

Aug 8, 2013·Journal of Cardiovascular Pharmacology·Nicola FerriAlberto Corsini
Apr 4, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Koteswara R ChavaGadiparthi N Rao
Oct 29, 2010·Virology Journal·Song ZhangGeorge Ruiz
Apr 17, 2009·Canadian Journal of Physiology and Pharmacology·Vsevolod A TkachukYelena V Parfyonova
Aug 27, 2016·International Journal of Molecular Sciences·Guan-Lin LeeCheng-Chin Kuo
May 31, 2018·Toxins·Lucie HénautZiad A Massy
Nov 19, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Toshihiro Ichiki
Apr 28, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Hideki OhtsuboKenji Sunagawa
Oct 25, 2017·Hypertension·Takashi MiyakeRyuichi Morishita
Sep 22, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Guan-Lin LeeCheng-Chin Kuo
Jul 31, 2013·Journal of Zhejiang University. Science. B·Li-na HanTing Zhang
Aug 1, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jeonghan Kim, Jesang Ko
Oct 10, 2013·The Journal of Biological Chemistry·Yongfeng LiuHarold A Singer
Oct 23, 2020·Canadian Journal of Physiology and Pharmacology·Vanessa TruongAshok K Srivastava

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.