cAMP-response element modulator-tau activates a distinct promoter element for the expression of the phospholipid hydroperoxide/sperm nucleus glutathione peroxidase gene

The Biochemical Journal
Federica TramerEnrico Panfili

Abstract

PHGPx (phospholipid hydroperoxide glutathione peroxidase) is a selenoprotein present in at least three isoforms in testis: cytosolic, mitochondrial and nuclear. All of these derive from the same gene and are structurally related with the exception of the snPHGPx (sperm nucleus-specific form), which differs from the others due to the presence of an arginine-rich N-terminus. It has been demonstrated recently that this N-terminus is encoded by an alternative exon located in the first intron of the PHGPx gene. The expression of snPHGPx has been attributed either to an alternative pre-mRNA splicing or to the presence of a distinct promoter region. Nevertheless, the exact molecular mechanism by which the expression of snPHGPx occurs has not been demonstrated so far. Preliminary sequence analysis of the region located upstream of the alternative exon revealed some potential DNA-binding sites, one of which is specific to the binding of CREM (cAMP-response element modulator) transcription factors. By using electrophoretic mobility-shift assays, we demonstrated that both nuclear protein extract from highly purified rat spermatid cells and recombinant CREM-tau protein can specifically bind to this element. Furthermore, we cloned a 1059 bp...Continue Reading

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Citations

Sep 29, 2009·The Journal of Biological Chemistry·Hirotaka ImaiYasuhito Nakagawa
Mar 16, 2013·Trends in Molecular Medicine·Thomas RauenGeorge C Tsokos
Aug 9, 2006·Molecular Reproduction and Development·Marianna RodovaGustavo Blanco
May 11, 2006·The Journal of Biological Chemistry·Astrid BorchertHartmut Kuhn
Dec 12, 2019·Antioxidants & Redox Signaling·Regina Brigelius-Flohé, Leopold Flohé
Apr 17, 2021·Reproductive Sciences·Marianna SantonastasoLucia Rocco
May 26, 2009·Biochimica Et Biophysica Acta·Zoia R Stoytcheva, Marla J Berry

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