cAMP signaling inhibits dihydropyridine-sensitive Ca2+ influx in vascular smooth muscle cells

Hypertension
S N OrlovP Hamet

Abstract

This study examines the role of the cAMP signaling pathway in the regulation of 45Ca influx in cultured vascular smooth muscle cells from the rat aorta. K+o-induced depolarization of smooth muscle cells increased the rate of 45Ca uptake by twofold to threefold. This effect was completely abolished by the dihydropyridine derivatives nifedipine and nicardipine, with a Ki of 3 and 10 nmol/L, respectively. Activators of cAMP signaling (isoproterenol, forskolin, cholera toxin) increased cAMP content by 50- to 100-fold and decreased voltage-dependent 45Ca uptake by 50% to 70%. Neither the dihydropyridines nor the cAMP activators affected basal 45Ca influx. Direct addition of the protein kinase inhibitor H-89 to the incubation medium in the 1- to 10-micromol/L range did not alter basal 45Ca uptake but completely abolished voltage-dependent Ca2+ transport. Preincubation of cells for 1 hour with 10 micromol/L H-89 did not modify basal and depolarization-induced 45Ca uptake in H-89-free medium but prevented forskolin-induced inhibition of voltage-dependent Ca2+ influx. The addition of cytoskeleton-active compounds reduced voltage-dependent Ca2+ transport and completely abolished its regulation by cAMP. Activation of cAMP signaling decrea...Continue Reading

References

Mar 1, 1992·Pflügers Archiv : European journal of physiology·U KlöcknerA Schwartz
Aug 1, 1992·The American Journal of Physiology·X L Chen, C M Rembold
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H KumeM I Kotlikoff
Dec 1, 1990·Pflügers Archiv : European journal of physiology·T N MarksS W Jones
Apr 1, 1989·Physiological Reviews·E K Hoffmann, L O Simonsen
Nov 24, 1987·European Journal of Pharmacology·J M Ousterhout, N Sperelakis
Dec 1, 1988·The American Journal of Physiology·R J McAnultyG J Laurent
Jan 1, 1994·Annual Review of Physiology·I B Levitan
Jun 1, 1995·The American Journal of Physiology·A G PratH F Cantiello
Jun 1, 1994·The American Journal of Physiology·J N LorenzN Sperelakis
Jul 1, 1994·Physiological Reviews·M E O'Donnell, N E Owen

❮ Previous
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Citations

Apr 12, 2012·Cellular and Molecular Life Sciences : CMLS·Scott GleimJohn Hwa
Aug 5, 2004·Pflügers Archiv : European journal of physiology·Yana J AnfinogenovaSergei N Orlov
Jun 30, 2001·Canadian Journal of Physiology and Pharmacology·N Thorin-TrescasesP Hamet
Dec 10, 1999·Canadian Journal of Physiology and Pharmacology·X WangW A Cupples
Jan 23, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Svetlana V KoltsovaSergei N Orlov
Nov 22, 2005·British Journal of Pharmacology·Ezequiel AlvarezFrancisco Orallo
Jun 1, 2008·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Kweon Young KimJae Yeoul Jun
Oct 30, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hai-Ying ZhangJoseph N Benoit
May 2, 2001·The Journal of Biological Chemistry·N O DulinT Voyno-Yasenetskaya
Nov 1, 2003·American Journal of Physiology. Cell Physiology·Aidar R GosmanovDonald B Thomason
Jul 25, 2017·American Journal of Physiology. Heart and Circulatory Physiology·Jimmy ToussaintDavid S Rumschitzki
Jul 23, 1998·Physiological Reviews·J D Stockand, S C Sansom
Jul 17, 1999·The American Journal of Physiology·M S Taylor, J N Benoit
Feb 7, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jennifer LaNickolai O Dulin
May 28, 2011·Physiological Research·M PintérováJ Zicha

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