PMID: 9445389Mar 7, 1998Paper

cAMP/phorbol ester response element is involved in transcriptional regulation of the human replacement histone gene H3.3B

The Biochemical Journal
O WittDetlef Doenecke

Abstract

The human histone H3.3B gene belongs to the group of replacement histone genes, which are up-regulated during differentiation of cells. Here we provide evidence that a cAMP response element/PMA response element (CRE/TRE) located in the proximal promoter contributes to the expression of the H3.3B gene. (1) Band shift and supershift analysis demonstrated the binding of AP-1 and transcription factors of the CRE-binding protein/activating-transcription-factor family to the H3.3B CRE/TRE. (2) Treatment of HeLa cells with PMA led to a 4-fold increase in H3. 3B mRNA levels within 2 h, whereas transcription of the cell cycle-dependent H3 histone genes remained constant. In contrast with PMA, cAMP did not affect H3.3B transcription. (3) PMA treatment of cells transiently transfected with H3.3B promoter constructs linked to a luciferase gene caused a 4-5-fold increase in reporter gene activity, whereas mutation of the CRE/TRE element abolished the PMA response. These results demonstrate that activation of the protein kinase C pathway by PMA results in an early up-regulation of H3.3B gene expression via the CRE/TRE element. Furthermore treatment with PMA apparently leads to differential induction of H3 histone subtype genes and this in tu...Continue Reading

Citations

Dec 3, 2004·Molecular Biology of the Cell·K HardyP S Jat
Mar 17, 2005·Bioinformatics·Rajesh ChowdharyVladimir B Bajic
Aug 5, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·John D LewisJuan Ausió
Sep 28, 2018·The Journal of Cell Biology·Shweta MendirattaGenevieve Almouzni
Mar 18, 2021·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Mudasir RashidSanjay Gupta

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