Campylobacter bacteriophage DA10: an excised temperate bacteriophage targeted by CRISPR-cas.

BMC Genomics
Steven HootonIan F Connerton

Abstract

Lytic bacteriophages that infect Campylobacter spp. have been utilized to develop therapeutic/decontamination techniques. However, the association of Campylobacter spp. and bacteriophages has been the focus of several strands of research aimed at understanding the complex relationships that have developed between predators and prey over evolutionary time. The activities of endogenous temperate bacteriophages have been used to evaluate genomic rearrangements and differential protein expression in host cells, and mechanisms of resistance to bacteriophage infection in campylobacters such as phase variation and CRISPR-mediated immunity. Temperate bacteriophage DA10 represents a novel excised and infective virus capable of replication in a restricted set of C. jejuni and C. coli hosts. Whole genome sequencing reveals that DA10 (35,379 bp) forms part of a novel group of temperate bacteriophages that have limited distribution among database host genome sequences. Analysis of potential host genomes reveals a robust response against DA10 and DA10-like bacteriophages is driven by CRISPR-mediated immunity with 75% of DA10 ORFs represented as ~ 30 bp spacer sequences in numerous Campylobacter Type II-C CRISPR arrays. Several DA10-like homo...Continue Reading

References

Oct 25, 1990·Nucleic Acids Research·T D Schneider, R M Stephens
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jul 1, 1970·Journal of Virology·J H BrynerD T Berman
Jan 8, 1999·Journal of Medical Microbiology·A D SailsA Curry
Aug 7, 2003·Applied and Environmental Microbiology·Robert J AtterburyIan F Connerton
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Harald BrüssowWolf-Dietrich Hardt
Jun 1, 2007·Nucleic Acids Research·Ibtissem GrissaChristine Pourcel
Aug 29, 2007·PLoS Pathogens·Andrew E ScottIan F Connerton
Feb 12, 2008·BMC Genomics·Ramy K AzizOlga Zagnitko
Mar 28, 2008·BMC Microbiology·Clifford G Clark, Lai-King Ng
Jan 20, 2009·Journal of Bacteriology·Esther J GaasbeekFimme J van der Wal
Jul 25, 2013·Archives of Virology·Muhammad Afzal JavedAndrew M Kropinski
Sep 21, 2013·Emerging Infectious Diseases·Benjamin FeodoroffHilpi Rautelin
May 6, 2014·Nature Structural & Molecular Biology·James K NuñezJennifer A Doudna
Sep 7, 2014·Genome Biology and Evolution·Rauni I KivistöMarja-Liisa Hänninen
Jun 13, 2015·Clinical Microbiology Reviews·Nadeem O KaakoushSi Ming Man
Jul 29, 2015·Frontiers in Microbiology·Helen L BrownArnoud H M van Vliet
Nov 26, 2015·Scientific Reports·C P A SkarpH Rautelin
Apr 14, 2016·Nature·Stineke van HouteEdze R Westra
May 5, 2016·Nucleic Acids Research·David ArndtDavid S Wishart
Mar 3, 2017·Journal of Clinical Microbiology·Ann-Katrin LlarenaMirko Rossi
Apr 12, 2017·Frontiers in Microbiology·Peter M O'Kane, Ian F Connerton
Apr 15, 2017·International Journal of Medical Microbiology : IJMM·C P A SkarpH Rautelin
Dec 21, 2017·Journal of Molecular Biology·Lukas ZimmermannVikram Alva
Jun 29, 2018·Frontiers in Cellular and Infection Microbiology·Yang HeLing Zhang
Nov 15, 2018·MBio·Nina Molin Høyland-KroghsboBonnie L Bassler
Feb 3, 2019·Applied and Environmental Microbiology·Anniina JaakkonenMarjaana Hakkinen
Jul 5, 2019·Clinical Microbiology Reviews·Daniela Costa, Gregorio Iraola

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Datasets Mentioned

BETA
GM

Methods Mentioned

BETA
electrophoresis
gene knockouts

Software Mentioned

CLC Genomics Workbench
BLASTp
BLAST
Gatan Microscopy Suite
mummer
BLASTn

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