Campylobacter jejuni enters gut epithelial cells and impairs intestinal barrier function through cleavage of occludin by serine protease HtrA

Gut Pathogens
Aileen HarrerSteffen Backert

Abstract

Campylobacter jejuni secretes HtrA (high temperature requirement protein A), a serine protease that is involved in virulence. Here, we investigated the interaction of HtrA with the host protein occludin, a tight junction strand component. Immunofluorescence studies demonstrated that infection of polarized intestinal Caco-2 cells with C. jejuni strain 81-176 resulted in a redistribution of occludin away from the tight junctions into the cytoplasm, an effect that was also observed in human biopsies during acute campylobacteriosis. Occludin knockout Caco-2 cells were generated by CRISPR/Cas9 technology. Inactivation of this gene affected the polarization of the cells in monolayers and transepithelial electrical resistance (TER) was reduced, compared to wild-type Caco-2 cells. Although tight junctions were still being formed, occludin deficiency resulted in a slight decrease of the tight junction plaque protein ZO-1, which was redistributed off the tight junction into the lateral plasma membrane. Adherence of C. jejuni to Caco-2 cell monolayers was similar between the occludin knockout compared to wild-type cells, but invasion was enhanced, indicating that deletion of occludin allowed larger numbers of bacteria to pass the tight ju...Continue Reading

References

Jan 1, 1997·Microbiology·Julian M Ketley
Mar 1, 1997·Trends in Microbiology·K G Wooldridge, J M Ketley
Dec 5, 2000·Molecular Biology of the Cell·M SaitouS Tsukita
Aug 17, 2002·Infection and Immunity·Guadalupe CortésSebastián Albertí
Mar 24, 2004·Current Opinion in Cell Biology·Roger VogelmannW James Nelson
Apr 12, 2005·Advanced Drug Delivery Reviews·Gemma J FeldmanMichael P Ryan
Apr 22, 2005·Biochimica Et Biophysica Acta·J D SchulzkeM Fromm
Jun 10, 2005·Journal of Biochemistry and Molecular Biology·Dong Young Kim, Kyeong Kyu Kim
Jul 8, 2005·FEMS Microbiology Letters·Mikael BrisslertMarianne Quiding-Järbrink
Dec 22, 2005·Infection and Immunity·Rebecca L WilsonDennis E Hruby
Feb 4, 2006·Cellular and Molecular Life Sciences : CMLS·I E BlasigJ Piontek
Jun 16, 2006·Current Opinion in Nephrology and Hypertension·Daniel Landau
Dec 7, 2006·The American Journal of Pathology·Jerrold R Turner
May 25, 2007·Cellular Microbiology·Malgorzata Krause-GruszczynskaSteffen Backert
Jun 19, 2007·FEMS Microbiology Letters·Malgorzata Krause-GruszczynskaSteffen Backert
Aug 19, 2007·Nature Reviews. Microbiology·Kathryn T YoungVictor J Dirita
Sep 5, 2008·Molecular Biology of the Cell·Junichi IkenouchiShoichiro Tsukita
Dec 9, 2008·Biochimica Et Biophysica Acta·Julian A Guttman, B Brett Finlay
Sep 17, 2009·Cellular and Molecular Life Sciences : CMLS·J K WalterIngolf E Blasig
Nov 16, 2010·Applied and Environmental Microbiology·Kristoffer T BaekLone Brøndsted
Feb 18, 2011·Nature Reviews. Molecular Cell Biology·Tim ClausenMichael Ehrmann
May 4, 2011·The Journal of Cell Biology·David R RaleighJerrold R Turner
Jun 23, 2012·Journal of Gastroenterology·Christian HirschSteffen Backert
Aug 25, 2012·Frontiers in Cellular and Infection Microbiology·Tadhg O Cróinín, Steffen Backert
Sep 29, 2012·Current Pharmaceutical Design·Joanna Skorko-GlonekBarbara Lipinska

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Citations

Jan 26, 2020·Letters in Applied Microbiology·D SimsonS Backert
Dec 5, 2019·Cell Communication and Signaling : CCS·Urszula ZarzeckaSteffen Backert
Apr 26, 2020·Applied and Environmental Microbiology·Samiullah KhanKapil K Chousalkar
May 5, 2020·European Journal of Microbiology & Immunology·Jakob KnorrNicole Tegtmeyer
Oct 22, 2020·Open Biology·Arturo Aguilar-RojasNancy Guillen
Dec 29, 2020·Frontiers in Cellular and Infection Microbiology·Irshad SharafutdinovSteffen Backert
Oct 17, 2020·PLoS Pathogens·Heba BarnawiDennis Linton
Dec 29, 2020·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Ruo-Yi XueHai-Bo Li
Apr 4, 2021·International Journal of Molecular Sciences·Tracy ParadisFabienne Bon
Nov 14, 2020·Applied Microbiology and Biotechnology·Vanessa KrelingAndreas Hensel
Jun 10, 2021·Microbiology Spectrum·Kourtney P NickersonChristina S Faherty
May 26, 2021·Infection and Immunity·Sean M CallahanJeremiah G Johnson
Aug 28, 2021·International Journal of Molecular Sciences·Fábia D Lobo de SáRoland Bücker

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Methods Mentioned

BETA
biopsies
transfection
electrophoresis
cleavage assay

Software Mentioned

Zeiss Image Examiner
ImageJ
PSIPRED
LAS AF
GraphPad Prism
- win64

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