Can chromatin conformation technologies bring light into human molecular pathology?

Acta biochimica Polonica
Marta Kubiak, Marzena A Lewandowska

Abstract

Regulation of gene expression in eukaryotes involves many complex processes, in which chromatin structure plays an important role. In addition to the epigenetic effects, such as DNA methylation and phosphorylation or histone modifications, gene expression is also controlled by the spatial organization of chromatin. For example, distant regulatory elements (enhancers, insulators) may come into direct physical interaction with target genes or other regulatory elements located in genomic regions of up to several hundred kilobases in size. Such long-range interactions result in the formation of chromatin loops. In the last several years, there has been a rapid increase in our knowledge of the spatial organization of chromatin in the nucleus through the chromosome conformation capture (3C) technology. Here we review and compare the original 3C and 3C-based methods including chromosome conformation capture-on-chip (4C), chromosome conformation capture carbon copy (5C), hi-resolution chromosome confomation capture (HiC). In this article, we discuss different aspects of how the nuclear organization of chromatin is associated with gene expression regulation and how this knowledge is useful in translational medicine and clinical applicat...Continue Reading

Citations

Jul 5, 2016·Nature Reviews. Nephrology·Marijn F StokmanAlbertien M van Eerde
Jul 26, 2016·Epigenetics & Chromatin·Kostas A TriantaphyllopoulosAndrew J Bannister
Jul 25, 2018·Journal of Thrombosis and Haemostasis : JTH·C Espitia JaimesM Neerman-Arbez
Jan 13, 2019·International Journal of Molecular Sciences·Marta KubiakMarzena Anna Lewandowska
Dec 14, 2018·Molecular Diagnosis & Therapy·Katarzyna KamińskaMarzena Anna Lewandowska

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