Can heparin immobilized surfaces maintain nonthrombogenic activity during in vivo long-term implantation?

ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs
C NojiriT Akutsu

Abstract

The authors previously demonstrated that heparin immobilized surfaces showed excellent nonthrombogenic properties for extracorporeal membrane oxygenation experiments as long as 168 hr. The characteristics of the heparin immobilized surfaces include high heparin bioactivity and prevention of platelet adhesion and complement activation. However, it is not known whether the heparin immobilized surfaces would be effective for in vivo long-term implantation. Heparin bioactivity may be lost because of complete degradation or blocking of binding sites on heparin by adsorbed proteins. This study attempted to elucidate the in vivo long-term fate of heparin immobilized surfaces. The blood contacting surfaces of the ventricular assist device (VAD) made from polyurethane was modified with heparin immobilization and evaluated in a long-term sheep left VAD (LVAD) model for as long as 3 months. After removal of the VAD, heparin bioactivity was measured by Factor Xa assay. The blood contacting surfaces were analyzed with a scanning electron microscope, and the adsorbed proteins on the surfaces of the diaphragm were analyzed by SDS-PAGE and Western blotting. The thickness of adsorbed proteins on the surfaces also was measured by a confocal lase...Continue Reading

Citations

Jul 2, 2009·Artificial Organs·Diyar Saeed, Kiyotaka Fukamachi
Jan 23, 1999·Archives of Physiology and Biochemistry·G W BosJ Feijen
Aug 17, 2002·Journal of Biomaterials Science. Polymer Edition·Jae Hyung Park, You Han Bae
Aug 3, 2001·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·L B SunN Kitamura
Feb 10, 2000·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·C NojiirT Akutsu
May 29, 2000·Journal of Biomedical Materials Research·E BryndaJ E Dyr

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