Abstract
The purpose of this study was to investigate the reproducibility of potential doubling time measurements of human tumors in different laboratories and to distinguish which steps in the measurement procedure were subject to the greatest variation. This was achieved by comparing measurements on the same source material in two different laboratories in which three aspects of the technique were separately studied, namely, preparation and staining of the nuclear suspensions, running the samples on the flow cytometer (FCM), and analyzing the two-parameter FCM data. This involved exchange between the two centers of fixed tumor material, stained nuclear suspensions, and FCM data on floppy disks. The analysis step was found to be the least variable step. For DNA synthesis time, Ts, and the labeling index, LI, the coefficients of determination (R2) ranged from 92% to 95.4%. A small systematic bias was observed, with one center measuring approximately 15% higher values for both LI and Ts. Different criteria for window placements were found to be a contributing factor. Variations in the FCM step were approximately equal to those for analysis, with no systematic deviations. Variations for the preparation and staining step were the largest (...Continue Reading
References
Aug 1, 1977·Injury·W M Steel
Nov 1, 1977·Annals of Neurology·E C Alvord
Dec 1, 1992·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·H K AwwadM S Zaghloul
Jan 1, 1992·International Journal of Radiation Oncology, Biology, Physics·C A LochrinM I Saunders
Apr 1, 1992·The British Journal of Surgery·D A RewG D Wilson
Jun 1, 1992·British Journal of Cancer·M H BennettJ H Laing
Jan 1, 1992·Cytometry·R A White, N H Terry
Nov 1, 1990·Cell and Tissue Kinetics·I E van OostrumD H Rutgers
Apr 1, 1988·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·A C BeggH Bartelink
Jan 1, 1988·Acta Oncologica·H R WithersB Maciejewski
Nov 1, 1985·Cytometry·A C BeggH Kärcher
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K HaustermansE van der Schueren
Dec 1, 1993·International Journal of Radiation Oncology, Biology, Physics·R CorvòV Vitale
Aug 1, 1993·International Journal of Radiation Oncology, Biology, Physics·J BourhisE P Malaise
Apr 1, 1993·British Journal of Cancer·M S WilsonP F Schofield
Citations
Jul 13, 2002·Cytometry·J-F MirjoletJ-L Merlin
Oct 1, 1995·Cytometry·W Schmidt
May 4, 1999·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·A C BeggG D Wilson
Feb 15, 2001·International Journal of Radiation Oncology, Biology, Physics·K Haustermans, J F Fowler
Feb 8, 2003·International Journal of Radiation Oncology, Biology, Physics·Werner DobrowskyGeorge D Wilson
Dec 1, 1996·International Journal of Radiation Oncology, Biology, Physics·P AntognoniM Danova
Apr 5, 2007·Nature Protocols·Nicholas H A Terry, R Allen White
Oct 1, 1996·Cell Proliferation·M C JohanssonS M Oredsson
Aug 31, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A SendlerJ R Siewert
Apr 3, 2007·Clinical Oncology : a Journal of the Royal College of Radiologists·G D Wilson
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K HaustermansE van der Schueren
Feb 7, 2001·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·D A Rew, G D Wilson
Jan 1, 1997·Radiation Oncology Investigations·H StruikmansJ J Battermann
Jan 15, 1999·British Journal of Cancer·G D WilsonP A Coucke
Nov 27, 1999·British Journal of Cancer·I Al-NabulsiK T Wheeler