Can measurements of potential doubling time (Tpot) be compared between laboratories? A quality control study

Cytometry
K HaustermansA C Begg

Abstract

The purpose of this study was to investigate the reproducibility of potential doubling time measurements of human tumors in different laboratories and to distinguish which steps in the measurement procedure were subject to the greatest variation. This was achieved by comparing measurements on the same source material in two different laboratories in which three aspects of the technique were separately studied, namely, preparation and staining of the nuclear suspensions, running the samples on the flow cytometer (FCM), and analyzing the two-parameter FCM data. This involved exchange between the two centers of fixed tumor material, stained nuclear suspensions, and FCM data on floppy disks. The analysis step was found to be the least variable step. For DNA synthesis time, Ts, and the labeling index, LI, the coefficients of determination (R2) ranged from 92% to 95.4%. A small systematic bias was observed, with one center measuring approximately 15% higher values for both LI and Ts. Different criteria for window placements were found to be a contributing factor. Variations in the FCM step were approximately equal to those for analysis, with no systematic deviations. Variations for the preparation and staining step were the largest (...Continue Reading

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Citations

May 4, 1999·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·A C BeggG D Wilson
Feb 15, 2001·International Journal of Radiation Oncology, Biology, Physics·K Haustermans, J F Fowler
Aug 31, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A SendlerJ R Siewert
Apr 3, 2007·Clinical Oncology : a Journal of the Royal College of Radiologists·G D Wilson
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K HaustermansE van der Schueren
Feb 7, 2001·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·D A Rew, G D Wilson

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