Can museum egg specimens be used for proteomic analyses?

Proteome Science
Steven J PortugalPhillip Cassey

Abstract

Mass spectrometry and proteomic analyses have become powerful tools for the analysis of proteins and peptides. Investigation of proteins contained in the various layers of the avian eggshell has focused entirely on domesticated species. It has been widely assumed that this existing research can inform the study of wild bird species despite the fact that the vast majority of the diversity in avian species (~95%) exists outside the Orders to which domestic and poultry species belong. Museum collections offer a potentially valuable source of material for studying composition of wild avian eggshell matrix proteins. We used museum and fresh eggshells of common quails Coturnix coturnix to compare the protein composition of their organic matrices. Four eggs of domestic chickens were analysed simultaneously as a control for comparison to the fresh and museum quail eggs. The determination of the proteins was carried out using enzymatic cleavage followed by high-performance mass spectrometry. We found that some of the expected key eggshell proteins (3 out of 11) were not present in the samples of museum quail egg. These proteins were either entirely absent from the museum eggs or the technique was unable to detect them. There was no patt...Continue Reading

References

Jul 1, 1992·British Poultry Science·M T HinckeR Narbaitz
Mar 26, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J L BadaH Hamilton
Aug 28, 1999·British Poultry Science·M PanheleuxY Nys
Mar 14, 2003·Nature·Ruedi Aebersold, Matthias Mann
May 13, 2005·Proceedings. Biological Sciences·M H SchweitzerS H Pincus
Nov 14, 2006·Biomacromolecules·Rajamani LakshminarayananSuresh Valiyaveettil
Dec 8, 2006·Proteomics·Karlheinz MannMatthias Mann

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Citations

May 13, 2011·Journal of the Royal Society, Interface·Branislav IgicMark E Hauber
Dec 29, 2015·Chemical Reviews·Sophie DallongevilleCaroline Tokarski
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Stephanie C McClellandSteven J Portugal
Sep 19, 2014·The Journal of Experimental Biology·Steven J PortugalPhillip Cassey

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