Can phosphorylation and dephosphorylation of rod outer segment membranes affect phosphatidate phosphohydrolase and diacylglycerol lipase activities?

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
M E Pérez RoqueN M Giusto

Abstract

Phosphatidate phosphohydrolase (PAPase) and diacylglycerol lipase (DGL) enzymatic activities were found to be differently affected by preincubation of rod outer segments (ROS) under protein phosphorylation or dephosphorylation conditions in darkness or in light. Under protein kinase C (PKC) phosphorylation conditions, PAPase and DGL were inhibited in darkness and in light. The inhibitory effect on PAPase and DGL activities by PKC phosphorylation in the presence of light was more pronounced when the activities were compared with the activities in control membranes determined in the presence of EGTA. The addition of PKC activators such as phorbol-12,13-dibutyrate and dioctanoylglycerol (DOG) instead of DG produced the same pattern of changes in enzymatic activities. Pretreatment of ROS membranes with cAMP-dependent protein kinase (PKA) produced a significant increase in both enzymatic activities in the presence of light. No changes were observed when ROS proteins were phosphorylated by PKA in the dark. Dephosphorylation of ROS membranes with alkaline phosphatase resulted in a decrease in PAPase activity that was more marked under light than under dark conditions. DGL activity was not modified under dephosphorylation conditions. T...Continue Reading

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Citations

Jul 19, 2000·Archives of Biochemistry and Biophysics·S J PasquaréN M Giusto
Jun 14, 2002·Archives of Biochemistry and Biophysics·P I Castagnet, N M Giusto
Jun 17, 2000·Progress in Lipid Research·N M GiustoM G Ilincheta de Boschero
Dec 17, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P I CastagnetN M Giusto
Oct 31, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Melina ReisenbergPatrick Doherty
Oct 16, 2009·Journal of Lipid Research·Norma M GiustoMónica G Ilincheta de Boschero
Oct 18, 2008·Neurochemistry International·S J Pasquaré, N M Giusto
Apr 15, 2011·Journal of Lipids·Susana J PasquaréNorma M Giusto
Mar 1, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zyanya P Espinosa-RiquerClaudia González-Espinosa
Jun 14, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Gerwyn MorrisAndre F Carvalho
Jan 11, 2011·Archives of Biochemistry and Biophysics·Virginia L GaveglioNorma M Giusto

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