Can the use of older-generation beta-lactam antibiotics in livestock production over-select for beta-lactamases of greatest consequence for human medicine? An in vitro experimental model

PloS One
Olanrewaju J OgunrinuH Morgan Scott

Abstract

Though carbapenems are not licensed for use in food animals in the U.S., carbapenem resistance among Enterobacteriaceae has been identified in farm animals and their environments. The objective of our study was to determine the extent to which older-generation β-lactam antibiotics approved for use in food animals in the U.S. might differentially select for resistance to antibiotics of critical importance to human health, such as carbapenems. Escherichia coli (E. coli) strains from humans, food animals, or the environment bearing a single β-lactamase gene (n = 20 each) for blaTEM-1, blaCMY-2, and blaCTX-M-* or else blaKPC/IMP/NDM (due to limited availability, often in combination with other bla genes), were identified, along with 20 E. coli strains lacking any known beta-lactamase genes. Baseline estimates of intrinsic bacterial fitness were derived from the population growth curves. Effects of ampicillin (32 μg/mL), ceftriaxone (4 μg/mL) and meropenem (4 μg/mL) on each strain and resistance-group also were assessed. Further, in vitro batch cultures were prepared by mixing equal concentrations of 10 representative E. coli strains (two from each resistance gene group), and each mixture was incubated at 37°C for 24 hours in non-an...Continue Reading

References

Dec 1, 1975·Journal of Pharmaceutical Sciences·C H NightingaleR Quintiliani
Nov 11, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C C Sanders, W E Sanders
Jan 1, 1987·Annual Review of Nutrition·E R Miller, D E Ullrey
Nov 1, 1985·Journal of Bacteriology·S E GeorgeT Melton
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·T J Case, M E Gilpin
May 16, 1980·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R P Ambler
Oct 1, 1995·Clinical Microbiology Reviews·D M Livermore
Oct 6, 1999·Current Opinion in Microbiology·D I Andersson, B R Levin
Feb 2, 2002·Applied and Environmental Microbiology·Thomas D LeserKristian Møller
May 4, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Scott A McEwen, Paula J Fedorka-Cray
Aug 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jian Xu, Jeffrey I Gordon
Dec 25, 2003·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
Feb 27, 2004·International Journal of Food Microbiology·Florent Baty, Marie-Laure Delignette-Muller
Apr 24, 2004·Canadian Journal of Microbiology·Jan Walther-Rasmussen, Niels Høiby
Dec 3, 2004·The Journal of Antimicrobial Chemotherapy·Elisabeth KugelbergDan I Andersson
May 6, 2005·The Journal of Antimicrobial Chemotherapy·Barry G Hall, Miriam Barlow
Sep 1, 2005·Current Opinion in Microbiology·Mark S WilkeNatalie C J Strynadka
Jun 3, 2006·The Journal of Infectious Diseases·James R JohnsonJavier Garau
Jul 6, 2006·Trends in Ecology & Evolution·Les DethlefsenDavid A Relman
Aug 11, 2007·Journal of Pharmaceutical and Biomedical Analysis·Salwa R El-ShabouryAzza H Rageh
Dec 19, 2007·Environmental Health Perspectives·Lance B PriceEllen Silbergeld
Jan 13, 2009·Clinical Microbiology Reviews·George A Jacoby
Mar 28, 2009·The Lancet Infectious Diseases·Patrice NordmannThierry Naas
Dec 8, 2009·International Journal of Food Microbiology·T W AlexanderT A McAllister
Dec 10, 2009·Antimicrobial Agents and Chemotherapy·Karen Bush, George A Jacoby
Jul 20, 2011·Antimicrobial Agents and Chemotherapy·Thierry NaasPatrice Nordmann
Jan 13, 2012·Journal of Clinical Microbiology·Mette V LarsenOle Lund
Mar 2, 2012·Emerging Infectious Diseases·Laurent PoirelPatrice Nordmann
Mar 29, 2012·The Journal of Antimicrobial Chemotherapy·Jennie FischerBeatriz Guerra
Apr 12, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Young Ah KimYohei Doi
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
Jun 29, 2012·Nature·Frank Aarestrup
Oct 5, 2012·The Journal of Antimicrobial Chemotherapy·Jennie FischerBeatriz Guerra
Mar 2, 2013·The Journal of Antimicrobial Chemotherapy·Wan-Jiang ZhangSiguo Liu
Mar 5, 2013·Nature Reviews. Microbiology·Daniel M Cornforth, Kevin R Foster
Mar 19, 2013·International Journal of Medical Microbiology : IJMM·Georgina Cox, Gerard D Wright
Mar 19, 2013·Frontiers in Microbiology·Gopi Patel, Robert A Bonomo
Apr 30, 2014·Antimicrobial Agents and Chemotherapy·Alessandra CarattoliHenrik Hasman
Sep 10, 2014·Annals of the New York Academy of Sciences·Elizabeth TemkinYehuda Carmeli
Nov 20, 2014·The Journal of Antimicrobial Chemotherapy·Charbel Al BayssariJean-Marc Rolain

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Software Mentioned

ABRicate
ResFinder
PlasmidFinder
[UNK]
SPAdes
SPAdes genome assembler Basespace
Stata
Velvet
SRST2ver

Related Concepts

Related Feeds

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.