Abstract
Prior models used to clarify which aspects of tissue microstructure mostly affect intracellular diffusion and corresponding diffusion-weighted magnetic resonance (DW-MR) signal have focused on relatively simple geometrical descriptions of the cellular microenvironment (spheres, randomly oriented cylinders, etc…), neglecting finer morphological details which may have an important role. Some types of neurons present high density of spines; and astrocytes and macroglial cells processes present leaflets, which may all impact the diffusion process. Here, we use Monte-Carlo simulations of many particles diffusing in cylindrical compartments with secondary structures mimicking spines and leaflets of neuronal and glial cell fibers, to investigate to what extent the diffusion-weighted signal of intracellular molecules is sensitive to spines/leaflets density and length. In order to study the specificity of DW-MR signal to these kinds of secondary structures, beading-like geometry is simulated as "control" deviation from smooth cylinder too. Results suggest that: a) the estimated intracellular tortuosity increases as spines/leaflets density or length (beading amplitude) increase; b) the tortuosity limit is reached for diffusion time td>20...Continue Reading
Citations
Feb 7, 2018·Frontiers in Neuroscience·Julien ValetteMarco Palombo
Dec 24, 2019·NMR in Biomedicine·Jan BrabecMarkus Nilsson
Jan 14, 2020·NMR in Biomedicine·Guglielmo GenoveseFrancesca Branzoli
Nov 7, 2019·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·David RomascanoAlessandro Daducci
Nov 5, 2020·Journal of Neuroscience Methods·Rafael N HenriquesAndrada Ianuş
Sep 4, 2020·NeuroImage·Zihan ZhouJianhui Zhong
Aug 18, 2020·NeuroImage·Hong-Hsi LeeDmitry S Novikov
Jul 6, 2020·NeuroImage·Ross CallaghanHui Zhang
Nov 13, 2020·Frontiers in Neuroscience·Kouhei KamiyaShigeki Aoki
Oct 24, 2020·Journal of Neuroscience Methods·Dmitry S Novikov
Dec 7, 2020·Journal of Neuroscience Methods·Hong-Hsi LeeDmitry S Novikov
Jul 22, 2020·Journal of Neuroscience Methods·Ileana O JelescuKurt G Schilling
Aug 20, 2020·Journal of Neuroscience Methods·Kouhei KamiyaShigeki Aoki
Mar 1, 2019·NeuroImage·Clémence LigneulJulien Valette
Nov 30, 2019·NeuroImage·Mélissa VincentJulien Valette
Jun 26, 2020·NeuroImage·Hong-Hsi LeeEls Fieremans
Jul 27, 2021·NeuroImage·A IanusM Palombo