PMID: 25798160Mar 24, 2015Paper

Canadian Public Health Laboratory Network laboratory guidelines for the use of direct tests to detect syphilis in Canada

The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale
Raymond S W TsangKamran Kadkhoda

Abstract

Treponema pallidum subsp. pallidum and/or its nucleic acid can be detected by various methods such as microscopy, rabbit infectivity test or polymerase chain reaction (PCR) tests. The rabbit infectivity test for T. pallidum, although very sensitive, has been discontinued from most laboratories due to ethical issues related to the need for animal inoculation with live T. pallidum, the technically demanding procedure and long turnaround time for results, thus making it impractical for routine diagnostic use. Dark-field and phase-contrast microscopy are still useful at clinic- or hospital-based laboratories for near-bedside detection of T. pallidum in genital, skin or mucous lesions although their availability is decreasing. The lack of reliable and specific anti-T. pallidum antibodies and its inferior sensitivity to PCR may explain why the direct fluorescent antibody test for T. pallidum is not widely available for clinical use. Immunohistochemical staining for T. pallidum also depends on the availability of specific antibodies, and the method is only applicable for histopathological examination of biopsy and autopsy specimens necessitating an invasive specimen collection approach. With recent advances in molecular diagnostics, P...Continue Reading

Citations

Feb 22, 2018·Sexually Transmitted Diseases·Michelle ShuelRaymond S W Tsang
Oct 12, 2017·Ocular Immunology and Inflammation·Parthopratim Dutta MajumderRupesh Agrawal
Nov 23, 2018·Journal of Medical Internet Research·Nitika Pant PaiRéjean Thomas
Aug 23, 2020·Indian Journal of Ophthalmology·S R RathinamGazal Patnaik
Aug 20, 2015·BMC Infectious Diseases·Susan Tuddenham, Khalil G Ghanem
Jan 14, 2021·Sexually Transmitted Infections·Dongdong LiChuanmin Tao
Feb 26, 2021·Frontiers in Cellular and Infection Microbiology·Yuting LuoYongjian Xiao
Nov 24, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Man-Li TongTian-Ci Yang
Nov 2, 2021·International Journal of STD & AIDS·Lauren Orser, Patrick O'Byrne

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