Cancer and embryo expression protein 65 promotes cancer cell growth and metastasis

Oncology Letters
Genglin JinChengchao Shou

Abstract

Cancer and embryo expression protein 65 (CEP65) is a centrosomal protein that is expressed at relatively high levels in embryonic tissue and different cancerous tissues, but its role in tumorigenesis remains unknown. In the present study, CEP65 was stably expressed in AGS gastric cancer cells. CEP65 was found to promote cell growth in the MTT assay and to enhance cell migration and invasion in Transwell chamber assays. To validate results from the in vitro experiments, CEP65 was stably expressed in BICR-H1 breast cancer cells through adenovirus-mediated transduction. By inoculating BICR-H1 cells on chick chorioallantoic membrane (CAM), it was found that CEP65 promotes cell growth on the CAM and increases cell metastasis to the lungs of the chicken. By utilizing a xenograft severe combined immunodeficiency mouse model, CEP65 was also found to accelerate BICR-H1 cell growth and metastasis to the lungs. Furthermore, it was shown that CEP65 increases matrix metalloproteinase (MMP)2 activity in zymographic assays, however, microarray screening and reverse transcription polymerase chain reaction validation revealed that CEP65 had no effect on the expression levels of MMP2 or MMP9, but decreased the expression levels of metastasis-ass...Continue Reading

References

Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T C HeB Vogelstein
Feb 13, 2001·Biochemical and Biophysical Research Communications·D Chen, C Shou
Jun 16, 2004·International Journal of Cancer. Journal International Du Cancer·Ping AnChengchao Shou
Oct 12, 2004·Biochemical and Biophysical Research Communications·Jianhui GuoChengchao Shou
Feb 3, 2005·The Journal of Biological Chemistry·Michiyo KoyanagiKunitada Shimotohno
Mar 12, 2005·Trends in Molecular Medicine·William G Stetler-Stevenson, Dong-Wan Seo
Sep 10, 2005·International Journal of Cancer. Journal International Du Cancer·Feng-qiang WangDavid A Fishman
Nov 16, 2007·Nature Reviews. Cancer·Kenji Fukasawa
Mar 27, 2012·Current Opinion in Structural Biology·Derek N WoolfsonAndrew R Thomson
Oct 16, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kazuhiro YamamotoHideaki Nagase
May 18, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Elin Hadler-OlsenLars Uhlin-Hansen
Jan 21, 2014·Connective Tissue Research·William G Stetler-Stevenson, Noah Veis Gavil

❮ Previous
Next ❯

Citations

Sep 19, 2020·Cancer Management and Research·Wenhui MoXuanfu Xu

❮ Previous
Next ❯

Datasets Mentioned

BETA
AF317887

Methods Mentioned

BETA
Protein Assay
chip
PCR
RAP

Software Mentioned

GenePix Pro
SPSS

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.