Cancer-associated differences in acetylcholinesterase activity in bronchial aspirates from patients with lung cancer

Clinical Science
Ana Martínez-López de CastroJuan Cabezas-Herrera

Abstract

In non-neuronal contexts, ACh (acetylcholine) is thought to be involved in the regulation of vital cell functions, such as proliferation, differentiation, apoptosis and cell-cell interaction. In airways, most cells express the non-neuronal cholinergic system, each containing a specific set of components required for synthesis, signal transduction and ACh hydrolysis. The aim of the present study was determine the expression of cholinergic system components in bronchial aspirates from control subjects and patients with lung cancer. We conducted an analysis of cholinergic components in the stored soluble and cellular fraction of bronchial aspirates from non-cancerous patients and patients diagnosed with lung cancer. The results show that the fluid secreted by human lung cells contains enough AChE (acetylcholinesterase) activity to control ACh levels. Thus these findings demonstrate that: (i) AChE activity is significantly lower in aspirates from squamous cell carcinomas; (ii) the molecular distribution of AChE in both bronchial cells and fluids consisted of amphiphilic monomers and dimers; and (iii) choline acetyltransferase, nicotinic receptors and cholinesterases are expressed in cultured human lung cells, as demonstrated by RT-...Continue Reading

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Citations

May 24, 2015·International Immunopharmacology·Ana Cristina Castillo-GonzálezJuan Cabezas-Herrera
Oct 5, 2010·The FEBS Journal·Encarnación Muñoz-DelgadoCecilio J Vidal
Aug 15, 2015·Thoracic Cancer·Hui-Jun XiZhao-Shen Li
Apr 17, 2016·Chemico-biological Interactions·F J CampoyS Nieto-Cerón
Apr 13, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Andreja LeskovacVesna Vasic

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