Cancer-associated Fibroblast-derived IL-6 Promotes Head and Neck Cancer Progression via the Osteopontin-NF-kappa B Signaling Pathway

Theranostics
Xing QinWantao Chen

Abstract

Osteopontin (OPN), a chemokine-like protein, plays a crucial role in the proliferation and metastasis of various cancers. However, how tumor stroma modulates the expression of neoplastic OPN and the multifaceted roles of OPN in head and neck cancer (HNC) are unclear. In this study, we tried to investigate the bridging role of OPN between tumor stroma and cancer cells. Methods: Immunohistochemical staining and quantitative real-time PCR were used to detect OPN expression in HNC tissues, and the correlations between OPN expression and clinicopathologic features were then analyzed. We used a co-culture assay to study the modulatory role of IL-6 on OPN expression and immunoprecipitation analysis was used to determine the endogenous interaction between OPN and integrin αvβ3. Furthermore, a xenograft assay was carried out to confirm the tumor-promoting role and the potential therapeutic value of OPN in HNC. Results: We found that OPN was significantly up-regulated in HNCs, and the elevated OPN was correlated with poor prognosis. Moreover, we identified IL-6 secreted by cancer-associated fibroblasts (CAFs) as the major upstream molecule that triggers the induction of neoplastic OPN. As such, during the interaction of fibroblasts and c...Continue Reading

Citations

Aug 21, 2019·Journal of Experimental & Clinical Cancer Research : CR·Yingying JiangWantao Chen
Sep 4, 2018·Clinical and Translational Medicine·Weilong ChenSuling Liu
Dec 20, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yawen LiBin Cheng
Apr 10, 2019·Journal of Experimental & Clinical Cancer Research : CR·Xiaoning WangWantao Chen
Aug 13, 2020·International Journal of Molecular Sciences·Cristiana TanaseMihail Eugen Hinescu
Jul 10, 2020·Cancer Research·Chengyao GuoZhenqiang Sun
Nov 27, 2020·Frontiers in Molecular Biosciences·Changsheng YangLujun Shen
Feb 9, 2021·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Chih-Yu PengShih-Shen Lin
Mar 7, 2021·International Journal of Molecular Sciences·Shang-Hung ChenJang-Yang Chang
Mar 14, 2021·Cancer Gene Therapy·Qinrong PingRuhong Li
Jun 11, 2021·Signal Transduction and Targeted Therapy·Fanglong WuHongmei Zhou
Jun 11, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yingying JiangJianjun Zhang
Aug 31, 2021·Frontiers in Cell and Developmental Biology·Hongyang LiuYinong Jiang

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Methods Mentioned

BETA
PCR
Infrared Imaging
ELISA
Assay
transfection
xenograft
ChIP
immunoprecipitation
co-IP
nuclear translocation

Software Mentioned

Pro Plus
Image
Statistical Package for Social Sciences

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